摘要
目的 了解胚胎期铅暴露对斑马鱼胚胎及幼鱼行为的影响,以探讨铅的神经行为发育毒性.方法 斑马鱼胚胎期暴露醋酸铅浓度分别为0、0.1、0.5、2.5和12.5 μmol/L,观察24 hpf胚胎的自主运动,分析测试5 dpf幼鱼对光周期的刺激反应.结果 各组胚胎自主运动观察结果依次为(5.22±1.02)、(6.25±1.46)、(6.12±0.92)、(5.46±0.92)、(4.62±0.81)次/min,与对照组比较,随着铅暴露浓度增加,胚胎自主运动次数呈高至低变化趋势,多组间差异有统计学意义(P＜0.05).幼鱼活力也随着铅暴露浓度增加呈现由高至低变化,明亮期12.5 μmol/L铅浓度组及黑暗期2.5 μmol/L铅浓度组平均活力均较对照组显著降低(P＜0.05);12.5 μmol/L铅浓度组亮转暗前后1 min活力改变量较对照组显著增高(P＜0.05),并且暗刺激后活力动态变化呈易激惹、易衰退状态.结论 胚胎期铅暴露能影响斑马鱼胚胎自主运动及幼鱼活力状态,低浓度铅暴露以兴奋作用为主,高浓度铅暴露则呈现抑制作用为主,但其内在机制尚有待进一步研究.
Abstract
Objective To investigate the effects of lead exposure during embryonic period on behavior of embryonic and larval zebrafish(Danio rerio),and explore the developmental neurobehavior toxicity of lead.Methods Zebrafish were exposed to lead acetate(PbAc)during embryonic period at the doses of 0,0.1,0.5,2.5 and 12.5 μmol/L,respectively,to observe spontaneous movement of embryos at 24 hpf(hours post fertilization),analyze and test the response to photoperiod stimulation in larval zebrafish at the time of 5 dpf(days post fertilization).Results The results of embryonic spontaneous movement were(5.22±1.02)、(6.25±1.46)、(6.12±0.92)、(5.46±0.92)、(4.62±0.81)(times/min),respectively.With the increasing of PbAc concentration,spontaneous movement varied from high to low,and there was a statistical significance(P＜0.05)among all the groups.The locomotor activity of larvae also changed from high to low with lead concentration increasing.To the control group,the average activity of the 12.5 μmol/L lead exposure group was decreased(P＜0.05)in the light period,and so did that of 2.5 μmol/L lead exposure group in the dark period.In the group of 12.5 μmol/L lead exposure,the activity variation in a minute before and after dark stimulation was increased significantly than that in the control group(P＜0.05),and the dynamic activity appeared irritable and then quickly declined.Conclusion Lead exposure during embryonic period can affect its later development of behavior such as embryonic spontaneous movement and larval locomotor activity.Low level lead exposure can improve facilitation in nervous system,whereas in the high level lead exposure,it appeared as mainly inhibitory effect due to damage increased,but the mechanism needs to be further studied.
NETL
NSTL
维普
万方数据