Exposure to Lead During Embryonic Period Impacts on Behavioral Development in Zebrafish
Objective To investigate the effects of lead exposure during embryonic period on behavior of embryonic and larval zebrafish(Danio rerio),and explore the developmental neurobehavior toxicity of lead.Methods Zebrafish were exposed to lead acetate(PbAc)during embryonic period at the doses of 0,0.1,0.5,2.5 and 12.5 μmol/L,respectively,to observe spontaneous movement of embryos at 24 hpf(hours post fertilization),analyze and test the response to photoperiod stimulation in larval zebrafish at the time of 5 dpf(days post fertilization).Results The results of embryonic spontaneous movement were(5.22±1.02)、(6.25±1.46)、(6.12±0.92)、(5.46±0.92)、(4.62±0.81)(times/min),respectively.With the increasing of PbAc concentration,spontaneous movement varied from high to low,and there was a statistical significance(P＜0.05)among all the groups.The locomotor activity of larvae also changed from high to low with lead concentration increasing.To the control group,the average activity of the 12.5 μmol/L lead exposure group was decreased(P＜0.05)in the light period,and so did that of 2.5 μmol/L lead exposure group in the dark period.In the group of 12.5 μmol/L lead exposure,the activity variation in a minute before and after dark stimulation was increased significantly than that in the control group(P＜0.05),and the dynamic activity appeared irritable and then quickly declined.Conclusion Lead exposure during embryonic period can affect its later development of behavior such as embryonic spontaneous movement and larval locomotor activity.Low level lead exposure can improve facilitation in nervous system,whereas in the high level lead exposure,it appeared as mainly inhibitory effect due to damage increased,but the mechanism needs to be further studied.
NETL
NSTL
万方数据
维普
