Investigate the effects of polysaccharides of Atractylodes macrocephala(PAMK)on the antioxidant capacity and apoptosis of gosling spleen after different times of LPS treatment.Eighty 1-day-old Magang geese were randomly divided into four groups,and the diet was supplemented with PAMK.2 mg/kg LPS was injected intraperitoneally on the 24th,26th and 28th day of age and spleen was collected 1 hour after the injection.The pathological changes of spleen tissue were observed.The mRNA expressions of spleen antioxidant enzymes(CAT,GSH-Px,SOD),apoptotic factors(Apaf-1,Caspase3,caspase9 and FAS)and anti-apoptotic factor BCL2 were detected by RT-PCR.PAMK can significantly protect the spleen of goslings from the tissue damage caused by LPS,and has a good anti-oxidation and alleviating the apoptosis of spleen cells effect.Among them,PAMK can restore antioxidant capacity of spleen treated by LPS,while PAMK has a certain lag effect on SOD.In addition,PAMK significantly increased the anti-apoptotic ability of spleen after LPS treatment 1,2 and 3 times,and the anti-apoptotic effect was most obvious after LPS treatment 3 times.
Polysaccharides of Atractylodes macrocephalaAntioxidantApoptosisSpleenGoose
维普
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