Regulation and Mechanism of cfa-miR-21 Carried by Canine Umbilical Cord Mesenchymal Stem Cell Exosomes on Proliferation and Migration of Canine Vascular Endothelial Cells
In order to study the mechanism of cfa-miR-21 carried by canine umbilical cord mesenchymal stem cell exosomes(UC-MSC-Exo)in promoting the proliferation and migration of canine vascular endothelial cells(VECs),the following studies were carried out in this experiment.In this study,canine VECs was used as a model.The best transfection scheme of cfa-miR-21 transfection reagent was screened by qRT-PCR,and the regulatory effect of cfa-miR-21 on cell proliferation and migration was detected by EdU cell proliferation imaging and scratch test.The target gene was predicted by bioinformatics analysis software,and the target gene was verified by dual luciferase reporter gene system and qRT-PCR.Then the expression of downstream ERK signal pathway protein was detected by Western Blot.The results suggest that cfa-miR-21 can promote the proliferation and migration of canine vascular endothelial cells,and cfa-miR-21 can exert its biological function by targeting SPRY1 and activating ERK signal pathway.This study offers new insights into the role of miRNAs in regulating angiogenesis,uncovering the functional mechanism of MSCs and potentially contributing to future drug development.
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