The South China tiger(Panthera tigris amoyensis),Bengal tiger(Panthera tigris tigris tigris),and Northeast tiger(Panthera tigris altaica)belong to the mammalian carnivorous order,feline leopard genus,and are first-class protected animals in China.In order to investigate the composition of gut microbiota in different tiger species in Guangdong Province,this study used 16S rDNA V1-V9 region third-generation sequencing to compare and annotate the gut microbiota of artificially captive tigers in Guangdong Province,and to explore their microbial composition and abundance.The results showed that the dominant phyla of gut microbiota in captive healthy tigers were Firmicutes,Actinobacteria,Proteobacteria,and Bacteroidetes;The gut microbiota of artificially captive South China tigers in Guangdong Province is relatively healthy,and there is no significant difference in the gut microbiota structure between different tiger species.However,due to conditions such as diet,environment,and status,there are still certain differences in the microbiota structure.The gut microbiota of Northeast tigers and Bengal tigers have some potential pathogenic genera with high abundance,indicating that the Bengal tigers and Northeast tigers investigated in this study have certain health risks,Need close attention.Our research has revealed the microbial community composition of different tiger species in Guangdong Province,which is of great significance for the comprehensive protection and rational captive breeding of this endangered species.