Fowl adenovirus 4(FAdV-4)is one of the common infectious pathogens in poultry,which can cause pericardial effusion and hepatitis syndrome in chickens with a mortality rate of 20%-80%,causing great economic losses to the poultry breeding industry.In this study,four strains of FAdV-4 were isolated and identified from Guangdong Province and Yunnan Province,respectively.Through whole gene amplification and sequence analysis of the Hexon and Fiber-2 genes of the main structural proteins of FAdV-4,the results showed that the nucleotide similarity of the Hexon gene and Fiber-2 gene of the four FAdV-4 isolates(named JM,QY,FS,and YN)was 100%.The phylogenetic tree of the Hexon and Fiber-2 genes showed that the four FAdV-4 isolates in this study were all located in species C of avian adenovirus group I,belonging to the branch of avian adenoviruses type 4 and were closely related to the prevalent FAdV-4 in China.This study provides a reference for exploring the genetic evolutionary relationship of the currently prevalent FAdV.
关键词
禽腺病毒4型/病毒分离/Hexon基因/Fiber-2基因/序列分析
Key words
Avian adenovirus type 4/Virus isolation/Hexon/Fiber-2/Sequence analysis
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