摘要
猪瘟(Classical swine fever,CSF)是由猪瘟病毒(Classical swine fever virus,CSFV)引起的猪的一种高度传染性和致死性疾病.该病呈世界性分布,在各国养猪产业中不同程度地流行,给养猪业造成巨大损失,是养猪业的主要问题之一.因此早期精准的抗原检测是有效控制CSF的先决条件.目前已研发出多个针对CSFV病毒抗原的检测方法,如:病原分离鉴定、荧光抗体试验、免疫组织化学技术、酶联免疫吸附试验、免疫层析技术、RT-PCR、实时定量RT-PCR、核酸等温扩增技术、微流控技术、基因芯片、CRISPR-Cas系统检测技术等.该文针对CS-FV的抗原检测技术和方法进行综述,以期为CSFV的临床监测和CSF的防控提供参考.
Abstract
Classical swine fever(CSF)is a highly contagious and fatal viral disease of pigs caused by Classical swine fever virus(CSFV).It is one of the major problems in pig industry because of its worldwide distribution and different degrees of prevalence in pig raising countries.Since the discovery of CSF,it has caused great losses to the pig industry.Therefore,high quality clinical diagnostic technology is a prerequisite for rapid detection and effective control of CSF.At present,a number of detection methods and technologies for CSFV antigen,protein and nucleic acid have been developed,such as:CSFV isolation and identification,Fluorescent antibody test(FAT),Immunohistochemical technology(IHC),Enzyme-linked immunosorbent assay(ELISA),Immunochromatographic assays(ICA),RT-PCR,Real-time quantitative RT-PCR(qRT-PCR),Nucleic acid isothermal amplification technology(NAIA),Microfluidic technology,Gene chip,CRISPR-Cas system technology and other methods.In this paper,CSFV antigen detection techniques and methods were reviewed,in order to provide reference for clinical monitoring of CSFV and prevention and control of CSF.
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