Preparation of Positive Reference in Duplex Fluorescent RT-PCR for detceting SVA and FMDV-O
To construct positive control samples for Seneca Valley virus(SVA)and foot-and-mouth disease virus type O(FMDV-O),positive reference sequences were designed based on selected target gene fragments.Armored RNA viral particles containing the target genes of FMDV-O and SVA were prepared through gene splicing and armored RNA technology.These particles were utilized as positive reference materials for SVA and FMDV-O.The results demonstrated that these positive control samples were non-infectious,faithfully simulated virus particle structures,were resistant to DNaseI and RNaseA degradation,exhibited high stability,and could be stored at-70℃for 18 months without significant changes in fluorescent reverse transcription polymerase chain reaction(RT-PCR)results.The prepared armored RNA viral particles containing the target genes of FMDV-O and SVA can be served as positive reference materials for quality control in the establishment of fluorescent RT-PCR detection methods for SVA and FMDV-O.
Seneca Valley virusFoot-and-Mouth disease virus type OMS2 bacteriophageArmored RNA technologyPositive reference materials
万方数据
维普
