Whole Genome Sequence Analysis of Six Goose-derived Newcastle Disease Viruses and their Pathogenicity to Goslings
To investigate the genetic evolution and pathogenicity of Newcastle Disease Virus(NDV)from geese in the Guangdong region,an experiment in 2019 involved the isolation and identification of goose-derived NDV from tissue specimens of suspected NDV-infected geese in Zhaoqing and Foshan areas.A strain with the smallest mean death time(MDT)was selected for pathogenicity testing in goslings.The results showed that six subtype Ⅻ b NDV strains from geese were isolated.These six strains had the highest nucleotide and amino acid sequence similarity between them,but showed lower similarity with genotype Ⅶ and commonly used vaccine strains like La Sota.Variations of different degrees were observed at some functional sites of the F protein and HN protein amino acid residues.Pathogenicity tests indicated that the subtypeⅫb strain Goose/GD/F424/2019 exhibited strong pathogenicity in goslings,with a high mortality rate of 87.5%,and displayed typical pathological changes such as splenomegaly with"branch-like"congestion,bleeding of proventricular spicules,bleeding of the duodenal mucosa,pancreatic degeneration and hemorrhage,and congestion and edema of brain tissue.The study indicated that all six isolated NDV strains from geese in the Guangdong region belonged to the Ⅻ b subtype and were highly pathogenic to goslings,laying a foundation for further research on the prevalence,evolution,and disease control of goose-derived NDV in the Guangdong area.
Goose Newcastle disease virusⅫ b sub-lineageGenome analysisPathogenicity
万方数据
维普
