Isolation,Identification and Drug Sensitivity Test of Pathogenic Klebsiella Pneumoniae from Elephant
In order to identify the pathogen and drug resistance that caused the illness of an Asian elephant,the experiment combined clinical observation to collect,isolate and purify the dominant pathogenic bacterium from the tail wound secretion of the Asian elephant,and carried out morphological observation,biochemical test,16S rRNA sequencing,virulence gene detection and drug sensitivity test.The Asian elephant showed symptoms such as poor spirit,loss of appetite,lying on the ground,hot exhalation and white secretion from the tail wound.The results of bacterial isolation and culture showed that the isolated strain was a milky white,smooth round gram-negative bacillus.The results of biochemical test showed that the bacterium was positive with glucose,citrate and lysine dehydrogenase.The sequence homology of the isolated strain 16S rRNA was more than 99.93%with 10 reference strains of Klebsiella pneumoniae,so it was identified as Klebsiella pneumoniae.Virulence gene test showed that the isolated strain carried six virulence genes,including kfu,uge,wabG,khe,ureA and fimH.The results of drug sensitivity test showed that the isolated strain was highly sensitive to enrofloxacin,levofloxacin and ofloxacin,and was resistant to 17 antibacterial drugs such as amoxicillin,cephalexin and butanacana,showing multiple drug resistance.After cage disinfection and medical treatment,the Asian elephant's condition improved and gradually recovered.
Asian elephantKlebsiella pneumoniaeIsolation and identificationVirulence genesDrug sensitivity test
万方数据
维普
