摘要
聚集蛋白聚糖基因(ACAN)是影响动物生长性状的重要候选基因,其编码的聚集蛋白聚糖对软骨和骨骼的结构形成与发展至关重要.该研究以狮头鹅、马岗鹅、乌鬃鹅为试验材料,利用混合DNA池并结合测序法筛查ACAN基因外显子区的多态位点,用PCR-RFLP和PCR产物直接测序的方法鉴定SNP多态位点基因型,并与乌鬃鹅、马岗鹅和狮头鹅的生长性状及狮头鹅屠体性状进行关联分析.结果在3个鹅群体ACAN基因外显子区共发现7个SNP多态位点,其中多态位点g.29647T＞C、g.31095C＞T、g.30246C＞T与胸深关联极显著,g.30135A＞G与胸深关联显著.多态位点g.29647T＞C、g.30135A＞G、g.30246C＞T与狮头鹅半净膛、腿肌、脚重关联显著,g.30246C＞T与脚重关联极显著,g.30085A＞G与脚重关联显著.本研究可为鹅分子标记辅助育种提供参考.
Abstract
The aggrecan gene(ACAN)is an important candidate gene influencing animal growth traits,and the aggrecan encoded by ACAN is crucial for the structural formation and development of cartilage and bone.In this study,Shitou geese,Magang geese,and Wuzong geese were used as experimental materials.A mixed DNA pool combined with sequencing was employed to screen polymorphic sites in the exon region of the ACAN.The genotypes of SNP polymorphic sites were identified using PCR-RFLP and sequencing of PCR products.Association analyses were then conducted between these SNPs and growth traits in Wuzong,Magang,and Shitou geese,as well as carcass traits in Shitou geese.A total of seven SNP polymorphic sites were identified in the exon region of the ACAN across the three goose populations in this study.Among them,the polymorphic sites g.29647T＞C,g.31095C＞T,and g.30246C＞T were highly significantly associated with breast depth,while g.30135A＞G was significantly associated with breast depth.The polymorphic sites g.29647T＞C,g.30135A＞G,and g.30246C＞T showed significantly associated with semi-eviscerated carcass weight,leg muscle weight,and foot weight in Shitou geese.In particular,g.30246C＞T was highly significantly associated with foot weight,and g.30085A＞G was significantly associated with foot weight.This study can provide some reference for molecular marker assisted breeding of geese.
维普
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