Isolation,Identification and Drug Resistance Analysis on a Strain of Cat-origin Proteus Mirabilis
In order to explore the cause of diarrhea in cats,a strain of bacterium was isolated from the anal swab sample of a cat with diarrhea.The isolated bacteria was identified by Gram staining,microscopic examination,motility growth test,16S rRNA gene amplification and sequencing.Hemolytic activity test,drug sensitivity test drug resistance gene detection and animal pathogenicity test were performed.The results showed that the isolated strain were Gram-negative short rods and displayed typical threaded migratory growth on both LB agar and blood agar media,consistent with the morphology and growth characteristics of Proteus mirabilis.The homology of the isolate with Proteus micrabilis(MW767054.1)from dogs in Genbank was 99.93％,which was finally confirmed as Proteus mirabilis.The isolated strain was not hemolytic,resistant to ceftraxone,cephalexin,enrofloxacin,ciprofloxacin,azithromycin,sulfamethoxazole,gentamicin,doxycycline and metronidazole.Five drug resistance genes CTX-M,aac C4,aac(6')-Ⅰ b-cr,sul Ⅰ and sul Ⅱ were detected in the isolated strain.Intraperitoneal injection of the isolated bacteria with 1×109 CFU/mL caused morbidity and death in mice.Astrain of Proteus mirabilis was successfully isolated from the diarrhea cats,which was pathogenic and showed widesprad drug resistance.
CatsProteus mirabilisIsolation and identificationDrug sensitivity testDrug resistance gene
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