Scamp2-/-/ApoE-/-小鼠繁殖及血清高密度脂蛋白胆固醇和肝脏三磷酸腺苷结合盒转运体A1水平测定
Breeding of Scamp2-/-/ApoE-/-mice and their detection of serum high density lipoprotein cholesterol and hepatic ABCA1 levels
洪伟涛 1陈耿基 1张鹏飞 2陈晓佳 1丁航 1马卫列 1张志珍1
作者信息
- 1. 广东医科大学基础医学院,广东东莞 523808
- 2. 深圳市龙华区中心医院中心实验室,广东深圳 518110
- 折叠
摘要
目的 繁殖Scamp2-/-/ApoE-/-小鼠,并测定其血清高密度脂蛋白胆固醇(HDL-C)及肝脏三磷酸腺苷结合盒转运体A1(ABCA1)水平.方法 将Scamp2-/-小鼠和ApoE-/-小鼠饲养于SPF级环境内,采用F0代纯合子杂交、F1代杂合子自交、F2代纯合子自交方法,繁殖获得Scamp2-/-/ApoE-/-小鼠,通过PCR扩增及琼脂糖凝胶电泳鉴定基因型.测定小鼠血清HDL-C及肝脏ABCA1 mRNA水平.结果 成功繁殖Scamp2-/-/ApoE-/-、Scamp2-/-/ApoE+/+和Scamp2+/+/ApoE-/-小鼠.与野生型小鼠相比,ApoE-/-、Scamp2-/-、Scamp2-/-/ApoE-/-小鼠血清HDL-C及肝脏ABCA1 mRNA水平均下降(P＜0.001);与ApoE-/-小鼠相比,Scamp2-/-/ApoE-/-小鼠血清HDL-C和肝脏ABCA1 mRNA水平分别降低了 25.26％和21.48％(P＜0.01).结论 成功繁殖获得Scamp2-/-/ApoE-/-小鼠;Scamp2敲除降低了小鼠肝脏ABCA1、血清HDL-C水平.
Abstract
Objective To breed Scamp2-/-/ApoE-/-mice and detect their serum high density lipoprotein cholesterol(HDL-C)and hepatic ABCA levels.Methods Scamp2-/-and ApoE-/-mice were raised in SPF room,and Scamp2-/-/ApoE-/-mice were bred using F0 homozygous hybridization and F1 heterozygous and F2 homozygous self-inbreeding.Genotypes of offspring were identified by PCR amplification and agarose gel electrophoresis.Serum HDL-C and hepatic ABCA1 mRNA levels were determined.Results Scamp2-/-ApoE-/-,Scamp2-/-/ApoE+/+and Scamp2+/+/ApoE-/-mice were successfully bred.Serum HDL-C and hepatic ABCA1 mRNA levels were lower in Scamp2-/-,Scamp2-/-and Scamp2-/-/ApoE-/-mice than in WT mice(P＜0.001).Compared with ApoE-/-mice,serum HDL-C and hepatic ABCA1 mRNA levels were respectively decreased by 25.26％and 21.48％in Scamp2-/-/ApoE-/-mice(P＜0.01).Conclusion Scamp2-/-/ApoE-/-mice are successfully obtained.Scamp2 knockout can reduce hepatic ABCA1 and serum HDL-C contents in mice.
关键词
Scamp2/ApoE/基因敲除/高密度脂蛋白胆固醇/ABCA1Key words
Scamp2/ApoE/gene knockout/high density lipoprotein cholesterol/ABCA1引用本文复制引用
基金项目
广东省基础与应用基础研究基金自然科学基金(2020A1515010224)
出版年
2024
NETL
NSTL
万方数据