摘要
目的 研究 5 种LED光质对莞香灵芝子实体生长发育及其主要活性成分的影响.方法 将不同光质的LED光源设为光照条件(红、蓝、黄、绿、白光为处理组,黑暗为黑暗组),对灵芝子实体进行培养,观察其生长速率、形态、多糖含量及三萜含量.结果 红、绿、黄、白、蓝 5 种光质处理组的灵芝子实体菌盖直径和干重均大于黑暗组(P<0.01或 0.05),黄光组的子实体干重最大(P<0.01 或 0.05),灵芝子实体出芝数和菌盖厚度与黑暗组比较差异无统计学意义(P>0.05).在灵芝的 5 个生长时期,蓝光组的多糖含量均明显高于其他各组(P<0.01);现蕾期、开伞期、弹孢前期,黄光组多糖含量高于除蓝光组外的其他各组(P<0.01);弹孢后期,白光组的多糖含量显著高于除蓝光组外的其他各组(P<0.01).芝盖形成期,红光组的灵芝三萜含量低于其他各组(P<0.01);弹孢前期,绿、白、红和蓝光组的灵芝三萜含量高于黑暗组(P<0.01 或 0.05).现蕾期、开伞期和弹孢后期,各组灵芝三萜含量比较差异无统计学意义(P>0.05).结论 5 种LED光质均影响莞香灵芝子实体的生长发育及其主要活性成分的生成,应根据各光质对灵芝子实体影响效果不同的特点合理安排光照.
Abstract
Objective To study the effects of different LED light qualities on the growth and development of Ganoderma lucidum substrates and their main active components.Methods Ganoderma lucidum substrates were cultured by setting LED light sources with different light qualities as light conditions,and the growth rate,morphology,polysaccharide content,and triterpene content were observed and measured as indicated methods.Results The diameter and dry weight of Ganoderma lucidum fruitum in red,green,yellow,white and blue light treatment groups were higher than those in dark group(P<0.01 or 0.05),and the dry weight of Ganoderma lucidum fruitum body in yellow light group was the largest(P<0.01 or 0.05).There was no statistical significance in the number of germination of Ganoderma lucidum fruitum and the thickness of fungus cap between the two groups(P>0.05).During the five growth periods of Ganoderma lucidum,the polysaccharide content of the blue light group was significantly higher than that of all other groups(P<0.01);during the bud-breaking stage,umbrella-opening stage,and pre-spores popping stage,the polysaccharide content of the yellow light group was higher than that of all other groups except the blue light group(P<0.01);and during the post-spores popping stage,the polysaccharide content of the white light group was significantly higher than that of all other groups except the blue light group(P<0.01).During the cap formation period,the triterpene content of Ganoderma lucidum in the red light group was lower than that in the other light quality groups and the dark group(P<0.01);during the pre-sporulation period,the triterpene content of Ganoderma lucidum was higher than that in the dark group in the green light,white light,red light and blue light groups(P<0.01 or 0.05).At the present bud stage,the opening stage and the late stage of spore popping,the difference of Ganoderma triterpene content between the groups was not statistically significant(P>0.05).Conclusion All five LED light qualities affected the growth and development of Ganoderma lucidum substrate and the production of its main active ingredients,and the light should be rationally arranged according to the characteristics of different effects of each light quality on Ganoderma lucidum substrate.
基金项目
2022年度广东省基础与应用基础研究基金自然科学基金项目(2022A1515012407)
广东省中医药科学技术研究项目(20221209)
2022年年东莞市社会发展科技(重点)项目(20221800905642)
广东省大学生创新创业训练计划项目(省-S202210571088)
广东省大学生创新创业训练计划项目(校-GDMU2021112)
广东医科大学校级大学生创新创业训练计划项目(GDMU2021138)
广东省基础与应用基础研究基金自然科学基金项目(2022A1515012407)
NETL
NSTL
万方数据