广东医科大学学报2024,Vol.42Issue(6) :590-598.

石榴果皮提取物纳米氧化铜抑菌纸的制备及性能研究

Preparation and property of nano-copper oxide antibacterial paper of pomegranate pericarp extract

黄杰锋 韩玉婷 余家琳 钟慕仪 程帆 钟国亮 钟文 刘建强
广东医科大学学报2024,Vol.42Issue(6) :590-598.

石榴果皮提取物纳米氧化铜抑菌纸的制备及性能研究

Preparation and property of nano-copper oxide antibacterial paper of pomegranate pericarp extract

黄杰锋 1韩玉婷 1余家琳 1钟慕仪 2程帆 1钟国亮 2钟文 3刘建强1
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作者信息

  • 1. 广东医科大学医用新材料研究与开发重点实验室//药学院,广东 东莞 523808
  • 2. 东莞市乳腺肿瘤精准诊疗与康复重点实验室//南方医科大学第十附属医院(东莞市人民医院)乳腺科,广东 东莞 523000
  • 3. 中国人民解放军第 32268 部队,云南 大理 671000
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摘要

目的 制备石榴果皮提取物的纳米氧化铜(CuO)抑菌纸并分析其性能.方法 采用索氏提取法提取突尼斯软籽石榴皮中的多酚类物质,利用石榴果皮中的主要多酚类物质安石榴苷与Cu2+产生共沉淀,使纳米CuO固定化;采用水热法制备具有金属+多酚类物质联合抗菌活性的纳米氧化铜抑菌纸;采用抑菌圈测试方法测试所合成抑菌纸的抗菌性能.结果 成功制备了基于石榴皮甲醇提取液(10 g石榴皮粉末+100 mL甲醇)纳米CuO抑菌纸.其中,抑菌性能最优制备方案为:所浸渍硫酸铜溶液浓度1.0 mol/L,浸渍温度60℃,浸渍时间600 s.此条件下合成的抑菌纸对大肠埃希菌、金黄色葡萄球菌均有较好的抑制作用,平均抑菌圈直径分别为(20.5±0.8)mm与(18.2±0.4)mm.结论 基于石榴皮甲醇提取液混合物所合成的纳米CuO抑菌纸对大肠埃希菌及金黄色葡萄球菌都有良好的抑菌效果.

Abstract

Objective To prepare nano-copper oxide(CuO)antibacterial paper from pomegranate peel extract and analyze its properties.Methods The polyphenols in Tunisian soft-seeded pomegranate pericarp were extracted by Soxhlete extraction method,and the main polyphenolic compound,punicalagin,was co-precipitated with Cu2+to immobilize nano-CuO.A nano-CuO antibacterial paper was prepared with antibacterial activity of metal and polyphenol substances using hydrothermal method.The antibacterial properties of the synthesized antibacterial paper were tested by inhibition circle test.Results The nano-CuO antibacterial paper was successfully prepared with methanol extract of pomegranate peel(10 g pomegranate peel powder+100 mL methanol).The optimal preparation scheme of antibacterial performance was:1.0 mol/L impregnated copper sulfate solution at 60℃for 600 s.The antibacterial paper synthesized under these conditions showed good inhibitory effects on Escherichia coli and Staphylococcus aureus,with average inhibition zone diameters of(20.5±0.8)mm and(18.2±0.4)mm,respectively.Conclusion The nano-CuO antibacterial paper synthesized from the mixture of pomegranate peel methanol extract has good antibacterial effects on E.coli and S.aureus.

关键词

石榴/多酚/安石榴苷/纳米氧化铜/抑菌纸

Key words

pomegranate/polyphenol/punicalagin/nano-copper oxide/antibacterial paper

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出版年

2024
广东医科大学学报
广东医学院

广东医科大学学报

影响因子:0.828
ISSN:1005-4057
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