Objective To establish a mouse model of aplastic anemia using oxaliplatin and X-ray irradiation.Methods Ten mice were randomly divided into control group(n=5)and model group(n=5).The model group were intraperitoneally injected with 3 mg/kg oxaliplatin at 1,3,5,7,9,11,13,15,17,and 19 days,and radiated with 3.0 Gy X-ray at 7 and 14 days;while the control group received intraperitoneal injection of the same dose of normal saline and sham irradiation with lead brick shielding at the same timepoints.The general condition and body weight of mice were observed.On the 20th day,blood examination,spleen index and pathological examination of spleen and bone marrow were performed.Results Compared with control group,model group showed listlessness after the first X-ray irradiation;dull fur color,gradual paleness of ears and limbs,and decreased weight growth rate after the second X-ray irradiation.The spleen volume and index decreased(P<0.001).White blood cells,red blood cells,hemoglobins,and platelets of peripheral blood reduced dramatically(P<0.001 or 0.01).Hematoxylin-eosin(HE)stain revealed loose structure of spleen and decreased nucleated cells with numerous fat vacuoles of bone marrow.Conclusion Combined oxaliplatin and X-ray irradiation can create an aplastic anemia model in mice.
维普
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