基于近红外水光谱组学的阿尔兹海默症诊断方法研究
Study on diagnosis methods of Alzheimer's disease based on near-infrared spectroscopy and aquaphotomics
曾薇 1黄文昌 1曾蕊 1黎远鹏 1黄富荣 2胡君辉1
作者信息
- 1. 广西师范大学物理科学与技术学院,广西桂林 541004
- 2. 暨南大学光电工程系,广东 广州 510632
- 折叠
摘要
阿尔兹海默症(Alzheimer's disease,AD)是一种不可逆转的中枢神经系统退形性疾病,针对目前临床上AD诊断方法存在的主观性强、耗时、有创等缺点,无创、准确性高、可实现实时诊断的近红外光谱(near-infrared spectroscopy,NIRS)分析有巨大优势.本文对采集的已确诊为AD和健康的265例血清样品光谱信息,进行光谱分析和主成分分析(principal component analysis,PCA),最后结合水光谱组学分析.结果表明:AD病人在PCA的前3个主成分分数中,PC1趋于负值,而健康人的PC1趋于正值.结合水光谱组学发现,AD患者在水光谱模式(water spectral pattern,WASP)为多氢键和OH拉伸处的吸光度比正常人高,血清中水的大分子团存在的概率更高.相反,正常人在WASP为单氢键、水合作用以及不含氢键的自由水处吸光度明显高于AD患者,血清中水的小分子团存在的概率更高.因此,基于近红外水光谱组学的AD诊断方法是可行的.
Abstract
Alzheimer's disease(AD)is an irreversible degenerative disease of the central nervous system.In view of the shortcomings of AD diagnosis methods,such as strong subjectivity,time-consuming,invasive,non-invasive,high accuracy,real-time diagnosis of near-infrared spectroscopy(NIRS)analysis has great advantages.In this paper,the spectral information of 265 serum samples that have been diagnosed as healthy and AD was collected,spectral analysis and principal component analysis(PCA)were carried out,and finally combined with aquaphotomics analysis.The results showed that in the first three PCA scores of patients with AD,PCI tended to be negative,which was different from that of healthy people.In combination with aquaphotomics analysis,it was found that the absorbance of AD patients at the place where the water spectral pattern(WASP)was multi-hydrogen bond and OH stretching was higher than that of normal people,and the probability of the existence of macromolecular groups of water in serum was higher.On the contrary,the absorbance of normal people in free water with single hydrogen bond,hydration and no hydrogen bond is significantly higher than that of AD patients,and the probability of small molecular groups of water in serum is higher.Therefore,the diagnosis method of AD based on NIRS combined with aquaphotomics is feasible.
关键词
阿尔兹海默症(AD)/近红外光谱(NIRS)/水光谱组学/主成分分析(PCA)Key words
Alzheimer's disease(AD)/near-infrared spectroscopy(NIRS)/aquaphotomics/principal component analysis(PCA)引用本文复制引用
出版年
2024
NETL
NSTL
万方数据