首页|A label-free lateral offset spliced coreless fiber MZI bio-sensor based on hydrophobin HGFI for TNF-α detec-tion

A label-free lateral offset spliced coreless fiber MZI bio-sensor based on hydrophobin HGFI for TNF-α detec-tion

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A real-time label-free lateral offset spliced coreless fiber(CF)Mach-Zehnder interferometer(MZI)biosensor func-tionalized with hydrophobin Grifola frondosa I(HGFI)was proposed for the detection of cytokine tumour necrosis factor alpha(TNF-α).The nanolayer self-assembled on the optical fiber surfaces by HGFI rendered the immobilization of probe TNF-α antibody and recognition of antigen TNF-α.Trifluoroacetic acid was utilized to remove the HGFI layer from the glass surface,which was validated by field emission scanning electron microscopy(FESEM)and water contact angle(WCA).Results demonstrated that the processes of HGFI modification,antibody immobilization and specific antibody detection can be monitored in real time.The proposed biosensor exhibited good specificity,repeat-ability and low detection limit for TNF-α,extending its application in inflammation and disease monitoring.

WANG Bo、DUAN Shaoxiang、ZHANG Hao、XU Haijin、LIU Bo、QIAO Mingqiang

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The Key Laboratory of Molecular Microbiology and Technology,Ministry of Education,Nankai University,Tianjin 300071,China

Institute of Modern Optics,Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology,Nankai University,Tianjin 300350,China

School of Life Science,Shanxi University,Taiyuan 030006,China

Sino-Swiss Scientific and Technological Cooperation Project supported by the Ministry of Science and Technology of China国家重点研发计划国家重点研发计划国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金天津市自然科学基金天津市自然科学基金天津市自然科学基金天津市自然科学基金Tianjin Education Commission Scientific Research ProjectTianjin Education Commission Scientific Research Project

2015DFG321402018YFA09001002018YFB180230211774181118042501190418011904262618750916210516419JCYBJC1670020JCQNJC0148021JCQNJC0021021JCYBJC000802018KJ1462019KJ016

2022

10.1007/s11801-022-2061-2

光电子快报(英文版)
天津理工大学

光电子快报(英文版)

EI
影响因子：0.641
ISSN：1673-1905
年,卷(期)：2022.18(5)
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