静电纺丝聚乙烯醇/丝素蛋白/秦皮提取物-聚己内酯双层纳米纤维膜的制备及抗氧化性能
Preparation of Electrospun Polyvinyl Alcohol/Silk Protein/Cortex Fraxini-Polycaprolactone Bilayer Nanofiber Film and Antioxidant Properties
黄丹 1杨安全 2谢敏 2王菁 2朱苗苗 1邓晏康 1陈龙 1熊燃华 3黄超伯3
作者信息
- 1. 南京林业大学化学工程学院,江苏南京 210037
- 2. 欧诗漫生物股份有限公司,浙江湖州 313000
- 3. 南京林业大学化学工程学院,江苏南京 210037;南京林业大学林业资源高效加工利用协同创新中心,江苏南京 210037
- 折叠
摘要
为制备干燥面膜,解决湿剂面膜中使用防腐剂、增稠剂等成分来延长面膜保质期对皮肤造成伤害的问题,通过顺序静电纺丝技术制备了以聚乙烯醇(PVA)、丝素蛋白(SF)负载秦皮提取物(CRF)为"亲肤层"和聚己内酯(PCL)为疏水层的双层纳米纤维膜.利用扫描电子显微镜、红外光谱分析及水接触角对纤维膜进行表征,并研究了纤维膜黏附性能、释放性能、抗氧化性能及生物相容性.结果表明,PVA/SF可以负载1%,3%和5%不同浓度的秦皮提取物,在湿润后PVA/SF/CRF-PCL纤维膜具有很强的黏附性,同时CRF可以在4 min左右释放平衡,达到快速释放的效果.负载5%秦皮提取物的PVA/SF纤维膜具有良好的抗氧化性能,对DPPH自由基和ABTS自由基清除率分别为89.13%±0.42%和85.49%±0.82%,PVA/SF/CRF-PCL纤维膜溶血率低,对B16细胞、Hela细胞均无毒性,B16细胞存活率均在89%以上,显示了良好的生物相容性,可以安全应用于皮肤领域.
Abstract
In order to prepare a dry mask and solve the problem of using preservatives and thickeners in wet mask to prolong the shelf life of mask and cause damage to skin,double-layer nanofiber films with polyvinyl alcohol(PVA),silk protein(SF)loaded with Cortex fraxini(CRF)as"skin-friendly layer"and polycaprolactone(PCL)as hydrophobic layer were prepared by sequential electrostatic spinning technology.The films were characterized by scanning electron microscopy,infrared spectroscopy and water contact angle,and their adhesion,release,antioxidant and biocompatibility properties were investigated.The results show that PVA/SF can be loaded with different concentrations of CRF at 1%,3% and 5%,and the PVA/SF/CRF-PCL fiber membranes have strong adhesion after wetting,while CRF can reach the release equilibrium at about 4 min to achieve rapid release.The PVA/SF fibrous membrane loaded with 5% CRF has good antioxidant properties,and the scavenging rates of DPPH radicals and ABTS radicals are 89.13%±0.42% and 85.49%±0.82%,respectively.The PVA/SF/CRF-PCL fibrous membrane has a low hemolysis rate and is not toxic to B16 cells and Hela cells,and the survival rate of B16 cells is 89% or more,showing a good biocompatibility and can be safely applied in the skin field.
关键词
静电纺丝/面膜/抗氧化/生物相容性Key words
electrostatic spinning/mask/antioxidant/biocompatibility引用本文复制引用
基金项目
国家自然科学基金(22275094)
国家自然科学基金(22275093)
国家自然科学基金(52203059)
江苏省自然科学基金(BK20220420)
出版年
2024
NETL
NSTL
万方数据