Extraction and Fraction of Polysaccharides from Mesona Chinensis Benth and Their Antioxygenic Property
Hot alkali extraction method combined with ultrasound technology was used to extract Mesona chinensis polysaccharide(MCP),then further graded polysaccharides MCP-1 and MCP-2 were also successfully prepared according to the principle of similar phase solubility.The effect of ultrasound time and extraction times on the extraction rate of Mesona chinensis polysaccharide were investigated by single-factor experiment.The results show that the optimal conditions for extracting MCP are 60 min of ultrasound time at 400 W and 4 times of alkali extraction,and the final yield of MCP is 44.02％±0.06％.MCP is characterized a kind of crude polysaccharide containing glyoxylate by UV and IR spectroscopy,and its average molecular weight(Mw)is 4.991 × 105 by gel permeation chromatography.In vitro antioxidant results show that compared with MCP,MCP-1 has stronger hydroxyl radical scavenging ability and MCP-2 has stronger superoxide anion radical scavenging ability,showing widely potential application in biomedical field.
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