凉粉草多糖提取和分级及抗氧化性能
Extraction and Fraction of Polysaccharides from Mesona Chinensis Benth and Their Antioxygenic Property
王治明 1谢慧红 1邱顺健 1游巧 1袁滢 1梁小兰 1刘红霞1
作者信息
- 1. 桂林理工大学材料科学与工程学院,广西桂林 541000
- 折叠
摘要
文中采用热碱提取法结合超声技术制备了凉粉草多糖(MCP),然后进一步根据相似相溶的原理对凉粉草多糖进行分级,成功获得了分级多糖MCP-1和MCP-2,并通过单因素实验研究了超声时间和提取次数对凉粉草多糖提取率的影响.结果表明,凉粉草多糖的最佳提取条件为在超声功率400 W时超声60 min,碱提取4次,在此条件下,多糖产率为44.02％±0.06％.紫外和红外光谱测试表明MCP是一种含糖醛酸的粗多糖,凝胶渗透色谱测试其重均分子量(Mw)为4.991× 105.而体外抗氧化实验表明,与MCP相比,MCP-1对羟基自由基的清除能力较强,MCP-2对超氧阴离子自由基的清除能力较强,在生物医药领域具有广阔的应用前景.
Abstract
Hot alkali extraction method combined with ultrasound technology was used to extract Mesona chinensis polysaccharide(MCP),then further graded polysaccharides MCP-1 and MCP-2 were also successfully prepared according to the principle of similar phase solubility.The effect of ultrasound time and extraction times on the extraction rate of Mesona chinensis polysaccharide were investigated by single-factor experiment.The results show that the optimal conditions for extracting MCP are 60 min of ultrasound time at 400 W and 4 times of alkali extraction,and the final yield of MCP is 44.02％±0.06％.MCP is characterized a kind of crude polysaccharide containing glyoxylate by UV and IR spectroscopy,and its average molecular weight(Mw)is 4.991 × 105 by gel permeation chromatography.In vitro antioxidant results show that compared with MCP,MCP-1 has stronger hydroxyl radical scavenging ability and MCP-2 has stronger superoxide anion radical scavenging ability,showing widely potential application in biomedical field.
关键词
凉粉草多糖/热碱提取法/超声/分级/自由基清除Key words
Mesona chinensis polysaccharide/hot alkali extraction/ultrasound/grade/radical scavenging引用本文复制引用
基金项目
广西自然科学基金资助项目(2023GXNSFAA026140)
广西科技基地和人才专项(2022AC18011)
国家自然科学基金资助项目(52263030)
出版年
2024
NETL
NSTL
万方数据