Synthesis and Analysis of Thermosensitive Hydrogels from Chitosan/k-Carrageenan Oligosaccharides
Taking advantage of the different charges of k-carrageenan oligosaccharide(KOS)and chitosan(CS),using KOS as a cross-linking agent,a thermosensitive hydrogel which can release KOS and control degradation can be prepared by ionic cross-linking glue.The CS acid solution and the KOS aqueous solution are mixed at room temperature to prepare a series of gel systems with the mass ratio of KOS to CS ranging from 2.25:1.0 to 2.75:1.0.The gel time is determined by the test tube inversion method,the volume change of phase transition is measured by the difference method,the swelling degree and degradation rate are measured by the weighing method,the sustained release of KOS from the gel is measured by the phenol-sulfuric acid method,and gel surface structure is observed under a scanning electron microscope(SEM).The adhesion and proliferation of central nervous immune effector cells(N9)on the gel were detected by MTT method.The results show that a series of KOS/CS hydrogels are temperature-responsive,appearing in a liquid sol state at 4 ℃,and can be phase-transformed into solid hydrogels at physiological temperature(37 ℃),and the gel time is 300±50-17±5 min.After gelation,there is obvious phase transition volume change,the porosity is(78.7±2.5)％-(92.9±2.1)％,the swelling rate is(558.7±5.046)％-(853.9±6.143)％,the degradation rate is(77.46±3.520)％-(70.68±4.080)％and the KOS sustained release degree is(30.38±0.9523)％-(24.24±3.988)％;the gel shows a fibrous porous structure under SEM,and supports the adhesion and proliferation of N9 cells.KOS/CS hydrogel has good temperature sensitivity,swelling property,controllable degradability,sustained release ability for KOS and cytocompatibility,which can provide a better biomaterial for the fields of drug delivery and tissue engineering.
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