Development of a Exopolysaccharides/9-Fluorenylmethyloxycarbonyl-phenylalanine Hydrogel System for Drug Delivery
This study introduces a new peptide-polysaccharide hydrogel for drug carriers,exploring its physical properties and drug release capabilities.Three variations of hydrogel EPS33,EPS50 and EPS66 with exopolysaccharides(EPS)contents of 33％,50％and 66％,respectively,were created using different ratios of 9-fluorenylmethoxycarbonyl-phenylalanine(Fmoc-F)to EPS.The influence of EPS on the structural,morphological,and mechanical characteristics of hydrogels was assessed using rheological,scanning electron microscopy,and textural analyses.The interaction between EPS and Fmoc-F within the hydrogels was examined by Fourier Transform Infrared(FTIR)spectroscopy.Furthermore,the effects of EPS on the biocompatibility,antioxidant properties,and drug delivery capabilities of the hydrogels were evaluated via hemolysis,free radical scavenging,drug loading,and in vitro release profiling using rhodamine B.The findings indicated that the Fmoc-F/EPS hydrogels with varying ratios of EPS displayed characteristics of gel-elastic solid behavior,with an observed increase in the network pore size with higher EPS content.The-OH groups of EPS were found to establish hydrogen-bonding interactions within and between Fmoc-F molecules.Compared to Fmoc-F,the hardness of EPS66 decreased to 1.689 g,and the loading capacity of rhodamine B increased by 22.79％to 259.8 mg/mL.The penetration depth through pig skin increased by 20.15％within 24 h,reaching 1576.5 μm.Therefore,it was demonstrated that Bifidobacterium EPS can create a stable hydrogel with a larger network pore size by engaging in hydrogen bonding interactions with Fmoc-F,resulting in an enhanced hydrophilic drug loading capacity.By manipulating the EPS ratio,novel peptide-polysaccharide hydrogels can be developed with superior drug-loading capacity,antioxidant properties,release capabilities,and biosafety,making them suitable for applications in skin hydrophilic drug delivery.
Self-assemblyPeptide-polysaccharide hydrogelsDrug carriersExopolysaccharidesSustained release of the drug
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