Preparation and Characterization of Decellularized Liver Matrix Composite Silk Fibroin Hydrogel Scaffold
In recent years,decellularized matrix has shown great potential in tissue engineering,but how to improve its mechanical properties is still a problem.In order to explore the potential of using a composite hydrogel scaffold with silk fibroin(SF)to improve the mechanical properties of decellularized liver matrix(DLM)for liver tissue engineering,four groups of SF/DLM composite scaffolds were prepared by cross-linking the rat liver with SF solution at the same concentration and different volume proportions.The proportions of SF to DLM solution were 100:0,75:25,50:50,25:75,respectively.The morphological structure,in vitro degradability,hydrophilicity,mechanical properties and cell compatibility of the scaffolds were characterized.The results showed that SF could improve the mechanical properties of the composite scaffold,the porosity,pressure resistance,degradability and hydrophilicity of the scaffold were all improved.Combined with the cell promoting function of DLM,the composite scaffold is expected to be applied in liver repair tissue engineering.
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