Abstract
Since Pb is a non-biodegradable inorganic pollutant and a non-essential metal,its long-term presence in soil poses a great threat to the environment.Iris lactea Pall.var.chinensis(Fisch.)Koidz.,a perennial dense bush herb with high resistance of Pb and wide adaptability,was used in pot experiments to study the effects of exogenous nitrate N(NO3--N)on the absorption and transportation of Pb and plant growth under different Pb concentrations.Then,the mechanism of NO3--N affecting Pb and nutrient uptake and transport was explored.The concentration of Pb in the experiment ranged from 0 to 1600 mg/kg,and the added concentration of NO3--N was 0.0-0.3 g/kg.The results showed that I.lactea was highly tolerant to Pb,and the shoot fraction was more sensitive to varied Pb concentrations in the soil than the root fraction.This protective function became more pronounced under the condition of raised Pb concentration in the soil.When the concentration of Pb in the soil reached 800 mg/kg,the highest Pb content of I.lactea was found under the condition of 0.1 g/kg of NO3--N addition.When Pb concentration in the soil increased to 1600 mg/kg,the increase in NO3--N addition promoted Pb uptake by the root.To ensure the well growth of I.lactea and the effect of remediation of Pb-contaminated soil,the recommended concentration of NO3--N in the soil is 0.1 g/kg.This result provides a theoretical basis for exogenous N regulation of phytoremediation of Pb-contaminated soil.
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