Characteristics of soil microbial communities structure and influencing factors in typical vegetation in the Beichuan River Source Area of Datong,Qinghai
This study aimed to investigate changes characteristics of soil microbial communities under typical vegetation in plateau forest ecosystems;a case study was undertaken the Beichuan River source area National Na-ture Reserve of Datong,Qinghai.This study analyzed 0-20 cm soil samples from six different vegetation species(Betula platyphylla,Populus cathayana,Picea crassifolia,Larix gmelinii var.principis-rupprechtii,Dasiphora fruticosa,and alpine meadow).The composition and diversity of soil microbial communities were determined us-ing Illumina NovaSeq sequencing,and soil physicochemical properties were measured via chemical analyses.The study investigated the correlation between soil microbial communities and soil physical and chemical proper-ties as well as identified factors driving the changes in soil microbial communities.The results revealed the pres-ence of 39 phyla,785 genera,and 1651 species of bacteria and 17 phyla,439 genera,and 559 species of fungi in the six vegetation samples.The total number of common microorganisms was lower than the specific one.The six vegetation soils were dominated by Proteobacteria,Actinobacteriota,and Acidobacteriota as well as by Basid-iomycota and Ascomycota fungal phyla.The bacterial communities of the six vegetation species exhibited signifi-cant differences in alpha diversity,with the highest diversity observed in the Populus cathayana Rehder bacterial community.Conversely,the fungal communities showed similarities,with the Betula platyphylla fungal commu-nity exhibiting the highest alpha diversity.The correlation between soil microbial communities and soil physico-chemical properties was analyzed.At the bacterial phylum level,a significant negative correlation was found be-tween soil pH and Desulfobacterota(P<0.05).Additionally,soil organic matter and total nitrogen contents were found to be highly significantly positively correlated with Ascomycota(P<0.01).At the fungal phylum level,a significant negative correlation was observed between soil pH and Entorrhizomycota(P<0.05).Soil organic mat-ter and total nitrogen contents were highly significantly positively correlated with Zoopagomycota(P<0.01)and significantly negatively correlated with Glomeromycota(P<0.05).Soil pH is a key environmental factor that af-fects the changes in soil bacterial and fungal communities.
microbial communitymicrobial diversityvegetation typeQinghai alpine region
万方数据
维普
