The purpose of this study was to explore the relationship between the soil fungal community structure and function and soil chemical properties and enzyme activity in Pinus tabuliformis sand-fixing forests of differ-ent ages,so as to provide a theoretical basis for the rational management and protection of P.tabuliformis sand-fixing forests.Taking the mobile sand of Horqin Sandy Land as the control(0 year),P.tabuliformis forests with sand fixation for 18,34,48 and 56 years were selected as the research objects,and high-throughput sequencing technology was used to analyze the differences in soil fungal community structure and functional groups.The re-sults show:(1)The 2517 OTUs obtained from the soil of the P.tabuliformis sand-fixing forest belong to 14 phy-la,48 classes,127 orders,286 families,and 579 genera of fungi.The dominant phyla were Ascomycota(47.91%-67.34%),Basidiomycota(18.45%-43.70%),and Mortierellomycota(1.41%-8.36%);the dominant genera were Biappendiculispora,Scleroderma,Tomentella,Knufia,and Amphinema.(2)Venn diagram and NMDS analysis showed that afforestation has a greater impact on soil fungal community structure.The ace index and chao index of soil fungi at each stand age increased significantly(P<0.05),and were related to organic matter,total nitrogen,and total phosphorus,urease,dehydrogenase,catalase,neutral phosphatase,sucrase and neutral protease have a significant positive correlation(P<0.05).(3)The fungal community was mainly composed of symbiotic and sap-rophytic types.After afforestation,the relative abundance of symbiotic types increased compared with the con-trol,while the relative abundance of saprophytic types was relatively stable.Afforestation plays an important reg-ulatory role on the structure and function of soil fungal communities.The research results enrich the research con-tent of soil microbial communities in sand-fixing forests and provide a basis for soil health evaluation of P.tabuli-formis sand-fixing forests in Horqin Sandy Land.
Horqin Sandy LandPinus tabuliformissoil fungicommunity structureecological functionstand age
维普
万方数据
