首页|Novelfunctionalproteinsinteractwith midkineinhepaticcancercells

Novelfunctionalproteinsinteractwith midkineinhepaticcancercells

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BACKGROUND:?Midkine? is? a? heparin-binding? growth?factor?that?promotes?the?proliferation,?survival,?migration?and?differentiation?of?various?target?cells.?Midkine?plays?an?important?role?in?tumorigenesis?and?tumor?progression,?and?is?overexpressed?in?many?human?malignant?tumors.?Patients?with? high? tumor? midkine? expression? frequently? have? a?worse?prognosis?than?those?with?low?expression.?The?present?study?was?designed?to?investigate?the?interaction?network?of?midkine?in?hepatic?cancer?cells,?and?to?elucidate?its?role?in?hepatocellular?carcinoma. <br> METHODS:?DNA?encoding?full-length?midkine?was?cloned?into?pDBLeu?vector?to?serve?as?bait?in?yeast?two-hybrid?screening?to?identify?interacting?proteins.?Candidate?proteins?were?examined?on?SC-Leu-Trp-His+3-AT?(20?mmol/L)?plates?and?assayed?for?X-gal?activity,?then?sequenced?and?classiifed?according?to?the?GenBank.?Finally,?identiifed?proteins?were?expressed?by?the?in vitro?expression?system?pCMVTnT,?and?protein?interactions?were?conifrmed?by?co-immunoprecipitation. <br> RESULTS:?Using?the?yeast?two-hybrid?system,?we?found?6?proteins?that?interacted?with?midkine:?NK-kappa-B?inhibitor?alpha? (I-κ-B-α),? Dvl-binding? protein? naked? cuticle? 2,?granulin,?latent?active?TGF-β?binding?protein?3,?latent?active?TGF-β binding? protein? 4,? and? phospholipid? scramblase?1.?In vitro?co-immunoprecipitation?demonstrated?that?all?identiifed?proteins?directly?interacted?with?midkine. <br> CONCLUSION:?The? identiifcation? of? midkine-interacting?proteins?in?hepatic?cancer?cells?indicates?that?midkine?is?a?multifunctional?factor?that?may?participate?in?cell?migration,?differentiation,?and?proliferation,?and?is?also?associated?with?the?multicellular?response?feedback?during?the?development?of?hepatocellular?carcinoma.

midkineyeast two-hybridinteractionhepatic cancer cell

Qiang Yan、Hui-Lian Huang、Xing Yao、Jing Li、Li-Qin Li、Jing Zhong、Li-Shan Min、Li-Cheng Dai and Shu-Sen Zheng

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Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital Yan Q and Yao X；Huzhou Key Laboratory of Molecular Medicine, Afifliated Central Hospital of Huzhou Teachers college Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC, Huzhou 313000, China；Division of Hepatobiliary and Pancreatic Surgery, First Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Yan Q and Zheng SS

Funding：This study was supported by grants from the National Natural Science Foundation of ChinaNatural Science Foundation of Huzhou

307725342010YS05

2012

10.1016/S1499-38721260160-X

国际肝胆胰疾病杂志(英文版)
浙江省医学学术交流管理中心,浙江大学医学院附属第一医院,浙江大学出版社有限责任公司

国际肝胆胰疾病杂志(英文版)

SCI
影响因子：0.668
ISSN：1499-3872
年,卷(期)：2012.(3)
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