首页|Polydatin ameliorates hepatic ischemia-reperfusion injury by modulating macrophage polarization

Polydatin ameliorates hepatic ischemia-reperfusion injury by modulating macrophage polarization

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  • 国家科技期刊平台
  • NETL
  • NSTL
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  • 维普
Background:Polydatin,a glucoside of resveratrol,has shown protective effects against various diseases.However,little is known about its effect on hepatic ischemia-reperfusion(I/R)injury.This study aimed to elucidate whether polydatin protects liver against I/R-induced injury and to explore the underlying mechanism.Methods:After gavage feeding polydatin once daily for a week,mice underwent a partial hepatic I/R pro-cedure.Serum alanine aminotransferase(ALT)/aspartate aminotransferase(AST),hematoxylin-eosin(H&E)and TdT-mediated dUTP nick-end labeling(TUNEL)staining were used to evaluate liver injury.The sever-ity related to the inflammatory response and reactive oxygen species(ROS)production was also investi-gated.Furthermore,immunofluorescence and Western blotting were used to detect macrophage polariza-tion and the NF-κB signaling pathway in macrophages.Results:Compared with the I/R group,polydatin pretreatment significantly attenuated I/R-induced liver damage and apoptosis.The oxidative stress marker(dihydroethidium fluorescence,malondialdehyde,su-peroxide dismutase and glutathione peroxidase)and I/R related inflammatory cytokines(interleukin-1β,interleukin-10 and tumor necrosis factor-α)were significantly suppressed after polydatin treat-ment.In addition,the result of immunofluorescence indicated that polydatin reduced the polarization of macrophages toward M1 macrophages both in vivo and in vitro.Western blotting showed that polydatin inhibited the pro-inflammatory function of RAW264.7 via down-regulating the NF-κB signaling pathway.Conclusions:Polydatin protects the liver from I/R injury by remodeling macrophage polarization via NF-κB signaling.

Hepatic ischemia-reperfusion injuryPolydatinMacrophagePolarizationInflammation

Hai-Li Bao、Chuan-Zhi Chen、Chang-Zhen Ren、Ke-Yan Sun、Hao Liu、Shao-Hua Song、Zhi-Ren Fu

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Department of Organ Transplantation,Shanghai Changzheng Hospital,Navy Military Medical University,Shanghai 200003,China

Department of Surgical Oncology,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China

Department of Cardiology,Shanghai Changzheng Hospital,Naval Military Medical University,Shanghai 200003,China

Department of General Surgery,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China

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National Natural Science Foundation of ChinaMedical Health Science and Technology Project of Health Commission of Zhejiang Province

819705632019RC055

2024

10.1016/j.hbpd.2022.08.009

国际肝胆胰疾病杂志(英文版)
浙江省医学学术交流管理中心,浙江大学医学院附属第一医院,浙江大学出版社有限责任公司

国际肝胆胰疾病杂志(英文版)

影响因子：0.668
ISSN：1499-3872
年,卷(期)：2024.23(1)
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