HSP110 aggravates ischemia-reperfusion injury after liver transplantation by promoting NF-KB pathway

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国家科技期刊平台
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外文摘要：Background:Ischemia-reperfusion injury(IRI)poses a significant challenge to liver transplantation(LT).The underlying mechanism primarily involves overactivation of the immune system.Heat shock protein 110(HSP110)functions as a molecular chaperone that helps stabilize protein structures.Methods:An IRI model was established by performing LT on Sprague-Dawley rats,and HSP110 was silenced using siRNA.Hematoxylin-eosin staining,TUNEL,immunohistochemistry,ELISA and liver en-zyme analysis were performed to assess IRI following LT.Western blotting and quantitative reverse transcription-polymerase chain reaction were conducted to investigate the pertinent molecular changes.Results:Our findings revealed a significant increase in the expression of HSP110 at both the mRNA and protein levels in the rat liver following LT(P＜0.05).However,when rats were injected with siRNA-HSP110,IRI subsequent to LT was notably reduced(P＜0.05).Additionally,the levels of liver enzymes and inflammatory chemokines in rat serum were significantly reduced(P＜0.05).Silencing HSP110 with siRNA resulted in a marked decrease in M1-type polarization of Kupffer cells in the liver and downregu-lated the NF-κB pathway in the liver(P＜0.05).Conclusions:HSP110 in the liver promotes IRI after LT in rats by activating the NF-κB pathway and in-ducing M1-type polarization of Kupffer cells.Targeting HSP110 to prevent IRI after LT may represent a promising new approach for the treatment of LT-associated IRI.

作者：

Qing-Zhi Hu、Zhen-Rui Cao、Wei-Xiong Zheng、Min-Jie Zhao、Jun-Hua Gong、Cong Chen、Zhong-Jun Wu、Rui Tao

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作者单位：

Department of Hepatobiliary Surgery,Bishan Hospital of Chongqing Medical University,Chongqing 402760,China

Department of Cardiothoracic Surgery,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China

Department of Hepatobiliary Surgery,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China

Department of Hepatobiliary Surgery,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China

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基金：

Natural Science Foundation of ChongqingNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaChongqing Research Program of Technological Innovation and Application Demonstration

项目编号：

CSTB2022NSCQ-MSX01488217066681873592cstc2021jscxgksbX0060

出版年：

2024

DOI：

10.1016/j.hbpd.2023.08.012

国际肝胆胰疾病杂志(英文版)

影响因子：0.668

ISSN：1499-3872

年,卷(期)：2024.23(4)

Qing-Zhi Hu,Zhen-Rui Cao,Wei-Xiong Zheng,et al.HSP110 aggravates ischemia-reperfusion injury after liver transplantation by promoting NF-KB pathway[J].国际肝胆胰疾病杂志(英文版),2024,23(4):344-352.DOI:10.1016/j.hbpd.2023.08.012.