首页|Comparison of biliary protein spectrum in gallstone patients with obesity and those with normal body weight
Comparison of biliary protein spectrum in gallstone patients with obesity and those with normal body weight
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国家科技期刊平台
NETL
NSTL
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Background:Obesity is a common public health issue and is currently deemed a disease.Research has shown that the risk of gallstones in individuals with obesity is elevated.This study aimed to explore the bile proteomics differences between cholelithiasis patients with obesity and normal body weight.Methods:Bile samples from 20 patients(10 with obesity and 10 with normal body weight)who under-went laparoscopic cholecystectomy at our center were subjected to tandem mass tag labeling(TMT)and liquid chromatography-tandem mass spectrometry(LC-MS/MS),followed by further bioinformatic analy-sis.Results:Among the differentially expressed proteins,23 were upregulated and 67 were downregulated.Bioinformatic analysis indicated that these differentially expressed proteins were mainly involved in cell development,inflammatory responses,glycerolipid metabolic processes,and protein activation cascades.In addition,the activity of the peroxisome proliferator-activated receptor(PPAR,a subfamily of nuclear receptors)signaling pathway was decreased in the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Two downregulated proteins in the PPAR signaling pathway,APO A-Ⅰ and APO A-Ⅱ,were confirmed using enzyme-linked immunosorbent assay.Conclusions:The PPAR signaling pathway may play a crucial role in the development of cholelithiasis among patients with obesity.Furthermore,biliary proteomics profiling of gallstones patients with obesity is revealed,providing a reference for future research.
Proteome profilingGallstonesObesity-associated gallstonesTandem mass tag labelingPPAR signaling
Min-Zhi Chen、Ping Xie、Xiao-Chang Wu、Zhen-Hua Tan、Hai Qian、Zhi-Hong Ma、Xing Yao
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Department of Hepatopancreatobiliary Surgery,Huzhou Central Hospital,Affiliated Huzhou Hospital,Zhejiang University School of Medicine,Huzhou 313000,China
Huzhou Key Laboratory of Molecular Medicine,Huzhou Central Hospital,Affiliated Huzhou Hospital,Zhejiang University School of Medicine,Huzhou 313000,China
PTM BioLab Co.Ltd.(Hangzhou,China)Public Welfare Research Fund of Huzhou City
国家科技期刊平台
NSTL
万方数据
