A novel saliva-based miRNA profile to diagnose and predict oral cancer

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国家科技期刊平台
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NSTL
维普
万方数据

外文摘要：Oral cancer(OC)is the most common form of head and neck cancer.Despite the high incidence and unfavourable patient outcomes,currently,there are no biomarkers for the early detection of OC.This study aims to discover,develop,and validate a novel saliva-based microRNA signature for early diagnosis and prediction of OC risk in oral potentially malignant disorders(OPMD).The Cancer Genome Atlas(TCGA)miRNA sequencing data and small RNA sequencing data of saliva samples were used to discover differentially expressed miRNAs.Identified miRNAs were validated in saliva samples of OC(n=50),OPMD(n=52),and controls(n=60)using quantitative real-time PCR.Eight differentially expressed miRNAs(miR-7-5p,miR-10b-5p,miR-182-5p,miR-215-5p,miR-431-5p,miR-486-3p,miR-3614-5p,and miR-4707-3p)were identified in the discovery phase and were validated.The efficiency of our eight-miRNA signature to discriminate OC and controls was:area under curve(AUC):0.954,sensitivity:86%,specificity:90%,positive predictive value(PPV):87.8%and negative predictive value(NPV):88.5%whereas between OC and OPMD was:AUC:0.911,sensitivity:90%,specificity:82.7%,PPV:74.2%and NPV:89.6%.We have developed a risk probability score to predict the presence or risk of OC in OPMD patients.We established a salivary miRNA signature that can aid in diagnosing and predicting OC,revolutionising the management of patients with OPMD.Together,our results shed new light on the management of OC by salivary miRNAs to the clinical utility of using miRNAs derived from saliva samples.

作者：

Jaikrishna Balakittnen、Chameera Ekanayake Weeramange、Daniel F.Wallace、Pascal H.G.Duijf、Alexandre S.Cristino、Gunter Hartel、Roberto A.Barrero、Touraj Taheri、Liz Kenny、Sarju Vasani、Martin Batstone、Omar Breik、Chamindie Punyadeera

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作者单位：

Saliva&Liquid Biopsy Translational Laboratory,Griffith Institute for Drug Discovery,Griffith University,Nathan,QLD,Australia

Department of Medical Laboratory Sciences,Faculty of Allied Health Sciences,University of Jaffna,Jaffna,Sri Lanka

Menzies Health Institute,Griffith University,Gold Coast,QLD,Australia

School of Biomedical Sciences,Faculty of Health,Queensland University of Technology,Brisbane,QLD,Australia

Centre for Cancer Biology,Clinical and Health Sciences,University of South Australia&SA Pathology,Adelaide,SA,Australia

Institute of Clinical Medicine,Faculty of Medicine,University of Oslo,Oslo,Norway

Department of Medical Genetics,Oslo University Hospital,Oslo,Norway

Griffith Institute for Drug Discovery,Griffith University,Nathan,QLD,Australia

QIMR Berghofer Medical Research Institute,Statistics Unit,Brisbane,QLD,Australia

School of Public Health,The University of Queensland,Brisbane,QLD,Australia

School of Nursing,Queensland University of Technology,Brisbane,QLD,Australia

eResearch,Research Infrastructure,Academic Division,Queensland University of Technology,Brisbane,QLD,Australia

Department of Anatomical Pathology,Royal Brisbane and Women's Hospital,Herston,QLD,Australia

Faculty of Medicine,The University of Queensland,Brisbane,QLD,Australia

Royal Brisbane and Women's Hospital,Cancer Care Services,Herston,QLD,Australia

Department of Otolaryngology,Royal Brisbane and Women's Hospital,Herston,QLD,Australia

Department of Oral and Maxillofacial Surgery,Royal Brisbane and Women's Hospital,Herston,QLD,Australia

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基金：

joint GUIPRS/AHEAD Scholarship and GU Postgraduate Research ScholarshipCancer AustraliaNational Health and Medical Research CouncilNational Health and Medical Research CouncilGarnett Passe and Rodney Williams FoundationRBWH Foundation

项目编号：

APP1145657APP 2002576APP 2012560NIH R21

出版年：

2024

DOI：

10.1038/s41368-023-00273-w

国际口腔科学杂志(英文版)

四川大学

国际口腔科学杂志(英文版)

CSTPCD

影响因子：0.684

ISSN：1674-2818

年,卷(期)：2024.16(1)

参考文献量52