首页|CD97 inhibits osteoclast differentiation via Rap1a/ERK pathway under compression

CD97 inhibits osteoclast differentiation via Rap1a/ERK pathway under compression

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 维普
  • 万方数据
Acceleration of tooth movement during orthodontic treatment is challenging,with osteoclast-mediated bone resorption on the compressive side being the rate-limiting step.Recent studies have demonstrated that mechanoreceptors on the surface of monocytes/macrophages,especially adhesion G protein-coupled receptors(aGPCRs),play important roles in force sensing.However,its role in the regulation of osteoclast differentiation remains unclear.Herein,through single-cell analysis,we revealed that CD97,a novel mechanosensitive aGPCR,was expressed in macrophages.Compression upregulated CD97 expression and inhibited osteoclast differentiation;while knockdown of CD97 partially rescued osteoclast differentiation.It suggests that CD97 may be an important mechanosensitive receptor during osteoclast differentiation.RNA sequencing analysis showed that the Rap1a/ERK signalling pathway mediates the effects of CD97 on osteoclast differentiation under compression.Consistently,we clarified that administration of the Rap1a inhibitor GGTI298 increased osteoclast activity,thereby accelerating tooth movement.In conclusion,our results indicate that CD97 suppresses osteoclast differentiation through the Rap1a/ERK signalling pathway under orthodontic compressive force.

Wen Wang、Qian Wang、Shiying Sun、Pengfei Zhang、Yuyu Li、Weimin Lin、Qiwen Li、Xiao Zhang、Zhe Ma、Haiyan Lu

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Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral Diseases,Hebei Medical University,Shijiazhuang,China

Department of Orthodontics,School and Hospital of Stomatology,Hebei Medical University,Shijiazhuang,China

State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology,Sichuan University,Chengdu,China

Department of Preventive Dentistry,School and Hospital of Stomatology,Hebei Medical University,Shijiazhuang,Hebei,China

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Natural Science Foundation of Hebei ProvinceHebei Province Science and Technology Support ProgramScience and Technology Research Project of Hebei Higher Education InstitutionsHighlevel Talent Funding Project of Hebei

H202020622618277756DZD2022010C20231141

2024

10.1038/s41368-023-00272-x

国际口腔科学杂志(英文版)
四川大学

国际口腔科学杂志(英文版)

CSTPCD
影响因子：0.684
ISSN：1674-2818
年,卷(期)：2024.16(1)
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