首页|Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer

Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer

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Cell-free RNA(cfRNA)allows assessment of health,status,and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases.Nevertheless,there is a lack of highly efficient and bias-free cfRNA isolation technologies due to the low abundance and instability of cfRNA.Here,we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids(containing cfRNA and viral RNA)in serum/plasma based on the inclusion of nucleic acids by metal-organic framework(MOF)materials,which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method.Importantly,the quality of cfRNA extracted by the MOF method is about 10-fold that of the kit method,and the MOF method isolates more than three times as many different RNA types as the kit method.The whole transcriptome mapping characteristics of cfRNA in serum from patients with liver cancer was described and a cfRNA signature with six cfRNAs was identified to diagnose liver cancer with high diagnostic efficiency(area under curve=0.905 in the independent validation cohort)using this MOF method.Thus,this new MOF isolation technique will advance the field of liquid biopsy,with the potential to diagnose liver cancer.

MOFcirculating cell-free RNAbiomarkerliver cancer diagnosis

Yuqing Sun、Haixin Yu、Shaoqing Han、Ruoxi Ran、Ying Yang、Yongling Tang、Yuhao Wang、Wenhao Zhang、Heng Tang、Boqiao Fu、Boshi Fu、Xiaocheng Weng、Song-Mei Liu、Hexiang Deng、Shuang Peng、Xiang Zhou

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College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers-Ministry of Education,Wuhan University,Wuhan 430072,China

Department of Digestive Surgical Oncology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

Department of Clinical Laboratory,Center for Gene Diagnosis and Program of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Wuhan 430071,China

College of Chemistry and Materials Science,Hubei Engineering University.Xiaogan 432000,China

Department of Pharmacology,School of Pharmacy,China Medical University,Shenyang 110122,China

Taikang Center for Life and Medical Sciences,Wuhan University,Wuhan 430071,China

Department of Hematology.Zhongnan Hospital of Wuhan University,Wuhan 430071,China

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National Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Natural science Foundation of ChinaNational Science Foundation for Young Scientists of ChinaHubei Provincial Innovation Group

9215330321721005919400008177227681902141219070782019CFA018

2024

10.1093/nsr/nwae022

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ISSN：
年,卷(期)：2024.11(1)
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