首页|Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes

Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes

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To investigate the circuit-level neural mechanisms of behavior,simultaneous imaging of neuronal activity in multiple cortical and subcortical regions is highly desired.Miniature head-mounted microscopes offer the capability of calcium imaging in freely behaving animals.However,implanting multiple microscopes on a mouse brain remains challenging due to space constraints and the cumbersome weight of the equipment.Here,we present TINIscope,a Tightly Integrated Neuronal Imaging microscope optimized for electronic and opto-mechanical design.With its compact and lightweight design of 0.43 g,TINIscope enables unprecedented simultaneous imaging of behavior-relevant activity in up to four brain regions in mice.Proof-of-concept experiments with TINIscope recorded over 1000 neurons in four hippocampal subregions and revealed concurrent activity patterns spanning across these regions.Moreover,we explored potential multi-modal experimental designs by integrating additional modules for optogenetics,electrical stimulation or local field potential recordings.Overall,TINIscope represents a timely and indispensable tool for studying the brain-wide interregional coordination that underlies unrestrained behaviors.

miniature microscopemultiple sitescalcium imagingmultimodal integrationhippocampus

Feng Xue、Fei Li、Ke-ming Zhang、Lufeng Ding、Yang Wang、Xingtao Zhao、Fang Xu、Danke Zhang、Mingzhai Sun、Pak-Ming Lau、Qingyuan Zhu、Pengcheng Zhou、Guo-Qiang Bi

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Department of Precision Machinery and Precision Instruments,University of Science and Technology of China,Hefei 230026,China

Hefei National Laboratory for Physical Sciences at the Microscale,University of Science and Technology of China,Hefei 230026,China

Interdisciplinary Center for Brain Information,the Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

Faculty of Life and Health Sciences,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China

School of Life Sciences,University of Science and Technology of China,Hefei 230026,China

Institute of Semiconductors,Chinese Academy of Sciences,Beijing 100083,China

Department of Modern Life Sciences and Biotecnology,Xiongan Institute of Innovation,Xiongan New Area,Xiongan 071899,China

Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen 518055,China

Suzhou Institute for Advanced Research,University of Science and Technology of China,Suzhou 215123,China

Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen 518055

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Strategic Priority Research Program of the Chinese Academy of SciencesNational Natural Science Foundation of China(NSFC)-Guangdong Joint FundNSFCKey-Area Research and Development Program of Guangdong ProvinceScience and Technology Innovation Committee of Shenzhen Municipality

XDB32030200U20A6005321009032018B030331001JCYJ20220818101611024

2024

10.1093/nsr/nwad294

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年,卷(期)：2024.11(1)
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