The microbiology related knowledge is a core theoretical system which should be grasped by the talent expert in Chinese baijiu brewing engineer,and also is an essential prerequisite for them to raise,analyze,and resolve the practical problems of production.However,the content of courses available could not be appropriated for the talent cultivation.Therefore,innovation and rebuilding to the current curriculum system should be performed.In this paper,we first extracted the theorical points potentially explaining the engineering problems,and then content of courses covering the points were selected.Finally,the new curriculum system was rebuilt from the selected courses and the available teaching methods and means.The new curriculum system was consisted of two parts,one was Microbiology as the core course,the other was co-curriculum covering six courses that aims to enhance foundation and promote innovation of the students.Under the background that innovating engineering curriculum system has been full carried out in domestic universities,it is expected that the exploration of Microbiology curriculum system and the course content reform of the Chinese baijiu brewing engineering major in the paper will have reference value for the construction of related majors in domestic universities.