Cultivating students'autonomous learning ability constitutes an essential objectiveof education.The blended teaching mode,which organically combines the traditional face-to-face teaching classroom with the modern Internet-based online learning,imposes higher demands on students and is conducive to fostering students'autonomous learning ability.This paper took Genetic Engineering course of the Brewing Engineering Department of Moutai Institute as an example.It was based on the problems existing in traditional teaching,with the aim of cultivating students'autonomous learning ability.By optimizing and improving online and offline teaching resources,reforming teaching approaches,and improving the evaluation system,a blended classroom that combined autonomous inquiry learning ofstudents and effective guidance of teachers was established.The results demonstrated that the blended teaching mode had improved students'learning enthusiasm,autonomous learning ability and course learning effect.This study can offer references for the teaching reform of genetic engineering-related courses.