The"Double First-Class"strategy aims to elevate a select group of universities and disciplines to the global top ranks.In this context,Petroleum Microbiology,as a vital interdisciplinary field,necessitates teaching approaches that cater to the practical needs of students and align with societal and economic development.This study investigates the significance,current status,and challenges of reforming Petroleum Microbiology education.Furthermore,it proposes methods and strategies for cultivating high-quality talents to address the evolving requirements of the petroleum industry.The teaching methods employed in Petroleum Microbiology courses not only fulfill students'practical application needs but also enhance their experimental and research capacities.Importantly,it fosters innovation by effectively integrating industry,academia,and research.