首页|Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation

Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation

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We present a novel time-of-flight resolved Bessel light bullet-enabled stimulated Raman scattering(B2-SRS)microscopy for deeper tissue 3D chemical imaging with high resolution without a need for mechanical z-scanning.To accomplish the tasks,we conceive a unique method to enable optical sectioning by generating the counter-propagating pump and Stokes Bessel light bullets in the sample,in which the group velocities of the Bessel light bullets are made ultraslow(e.g.,vg ≈ 0.1 c)and tunable by introducing programmable angular dispersions with a spatial light modulator.We theoretically analyze the working principle of the collinear multicolor Bessel light bullet generations and velocity controls with the relative time-of-flight resolved detection for SRS 3D deep tissue imaging.We have also built the B2-SRS imaging system and present the first demonstration of B2-SRS microscopy with Bessel light bullets for 3D chemical imaging in a variety of samples(e.g.,polymer bead phantoms,biological samples such as spring onion tissue and porcine brain)with high resolution.The B2-SRS technique provides a＞2-fold improvement in imaging depth in porcine brain tissue compared to conventional SRS microscopy.The method of optical sectioning in tissue using counter-propagating ultraslow Bessel light bullets developed in B2-SRS is generic and easy to perform and can be readily extended to other nonlinear optical imaging modalities to advance 3D microscopic imaging in biological and biomedical systems and beyond.

Shulang Lin、Li Gong、Zhiwei Huang

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Optical Bioimaging Laboratory,Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore 117576,Singapore

Academic Research Fund(AcRF)from the Ministry of Education(MOE)Academic Research Fund(AcRF)from the Ministry of Education(MOE)Academic Research Fund(AcRF)from the Ministry of Education(MOE)Academic Research Fund(AcRF)from the Ministry of Education(MOE)Merlion Fund2024Tsinghua-NUS Joint Research Initiative FundNational Medical Research Council(NMRC),SingaporeNational Medical Research Council(NMRC),Singapore

A-8000117-01-00R397-000-334-114R397-000-371-114R397-000-378-114R397-000-356-133A-0009502-01-00A-8001143-00-00

2024

10.1038/s41377-024-01498-y

光:科学与应用(英文版)
中国科学院长春光学精密机械与物理研究所

光:科学与应用(英文版)

CSTPCD
ISSN：2095-5545
年,卷(期)：2024.13(7)