Extended-depth of field random illumination microscopy,EDF-RIM,provides super-resolved projective imaging

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外文摘要：The ultimate aim of fluorescence microscopy is to achieve high-resolution imaging of increasingly larger biological samples.Extended depth of field presents a potential solution to accelerate imaging of large samples when compression of information along the optical axis is not detrimental to the interpretation of images.We have implemented an extended depth of field(EDF)approach in a random illumination microscope(RIM).RIM uses multiple speckled illuminations and variance data processing to double the resolution.It is particularly adapted to the imaging of thick samples as it does not require the knowledge of illumination patterns.We demonstrate highly-resolved projective images of biological tissues and cells.Compared to a sequential scan of the imaged volume with conventional 2D-RIM,EDF-RIM allows an order of magnitude improvement in speed and light dose reduction,with comparable resolution.As the axial information is lost in an EDF modality,we propose a method to retrieve the sample topography for samples that are organized in cell sheets.

作者：

Lorry Mazzella、Thomas Mangeat、Guillaume Giroussens、Benoit Rogez、Hao Li、Justine Creff、Mehdi Saadaoui、Carla Martins、Ronan Bouzignac、Simon Labouesse、Jérome Idier、Frédéric Galland、Marc Allain、Anne Sentenac、Loïc LeGoff

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作者单位：

Aix Marseille Université,CNRS,Centrale Med,Institut Fresnel UMR7249,Turing Center for Living Systems,Marseille,France

LITC Core Facility,Centre de Biologie Integrative(CBI),CNRS,Université de Toulouse,UT3,Toulouse,France

MCD,Centre de Biologie Intégrative(CBI),CNRS,Université de Toulouse,UT3,Toulouse,France

Aix Marseille University,CNRS,IBDM UMR7288,Turing Centre for Living Systems,Marseille,France

LS2N,CNRS UMR 6004,F44321 Nantes Cedex 3,France

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出版年：

2024

DOI：

10.1038/s41377-024-01612-0

光:科学与应用(英文版)

中国科学院长春光学精密机械与物理研究所

光:科学与应用(英文版)

CSTPCD

ISSN：2095-5545

年,卷(期)：2024.13(12)