首页|Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer

Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured silica overlayer

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
We demonstrate long-range enhancement of fluorescence and Raman scattering using a dense random array of Ag nanoislands(AgNIs)coated with column-structured silica(CSS)overlayer of over 100 nm thickness,namely,remote plasmonic-like enhancement(RPE).The CSS layer provides physical and chemical protection,reducing the impact between analyte molecules and metal nanostructures.RPE plates are fabricated with high productivity using sputtering and chemical immersion in gold(l)/halide solution.The RPE plate significantly enhances Raman scattering and fluorescence,even without proximity between analyte molecules and metal nanostructures.The maximum enhancement factors are 107-fold for Raman scattering and 102-fold for fluorescence.RPE is successfully applied to enhance fluorescence biosensing of intracellular signalling dynamics in HeLa cells and Raman histological imaging of oesophagus tissues.Our findings present an interesting deviation from the conventional near-field enhancement theory,as they cannot be readily explained within its framework.However,based on the phenomenological aspects we have demonstrated,the observed enhancement is likely associated with the remote resonant coupling between the localised surface plasmon of AgNIs and the molecular transition dipole of the analyte,facilitated through the CSS structure.Although further investigation is warranted to fully understand the underlying mechanisms,the RPE plate offers practical advantages,such as high productivity and biocompatibility,making it a valuable tool for biosensing and biomolecular analysis in chemistry,biology,and medicine.We anticipate that RPE will advance as a versatile analytical tool for enhanced biosensing using Raman and fluorescence analysis in various biological contexts.

Takeo Minamikawa、Reiko Sakaguchi、Yoshinori Harada、Hiroki Tanioka、Sota Inoue、Hideharu Hase、Yasuo Mori、Tetsuro Takamatsu、Yu Yamasaki、Yukihiro Morimoto、Masahiro Kawasaki、Mitsuo Kawasaki

展开 >

Department of Systems Innovation,Graduate School of Engineering Science,Osaka University,Osaka 560-8531,Japan

Division of Interdisciplinary Researches for Medicine and Photonics,Institute of Post-LED Photonics,Tokushima University,Tokushima 770-8506,Japan

Department of Pathology and Cell Regulation,Graduate School of Medical Science,Kyoto Prefectural University of Medicine,Kyoto 602-8566,Japan

PRESTO,Japan Science and Technology Agency(JST),Tokushima 770-8506,Japan

institute for Integrated Cell-Material Sciences(iCeMS),Kyoto University,Kyoto 606-8501,Japan

Department of Synthetic Chemistry and Biological Chemistry,Graduate School of Engineering,Kyoto University,Kyoto 615-8510,Japan

Department of Medical Photonics,Graduate School of Medical Science,Kyoto Prefectural University of Medicine,Kyoto 602-8566,Japan

Technology and Engineering Division,Ushio Inc.,Hyogo 671-0224,Japan

The Institute of Science and Industrial Research,Osaka University,Osaka 567-0047,Japan

Department of Molecular Engineering,Graduate School of Engineering,Kyoto University,Kyoto 615-8510,Japan

展开 >

2024

10.1038/s41377-024-01655-3

光:科学与应用(英文版)
中国科学院长春光学精密机械与物理研究所

光:科学与应用(英文版)

CSTPCD
ISSN：2095-5545
年,卷(期)：2024.13(12)