Objective To observe the effect of compound Xinshuaikang capsule on the expression of SERCA2a and cardiac function in rats with heart failure.Methods A total of 120 male SD rats with SPF grade were selected and divided into blank group(n=20)and model group(n=100).The rat heart failure model was constructed by adriamycin intraperitoneal injection.After successful modeling,the rats(n=91)were randomly divided into model group(n=19),digoxin group(n=18),compound Xinshuaikang low-dose group(n=18),medium-dose group(n=18)and high-dose group(n=18).Cardiac function indexes,SERCA2a and its mRNA gene expression levels were detected in each experimental group.Results Compared with blank group,the expression levels of LVEDD,LVFS,NT-proBNP and miR-25 were significantly increased in model group,digoxin group and Compound Xinshuaikang low-middle-high dose group,while the expression levels of SERCA2a,its mRNA and LVEF were significantly decreased(P＜0.05).Compared with model group,the expression levels of LVEDD,LVFS,NT-proBNP and miR-25 were significantly decreased in compound Xinshuaikang low-medium-high-dose group and digoxin group,while SERCA2a and its mRNA expression levels and LVEF were significantly increased(P＜0.05).Conclusion Compound Xin-shuaikang capsule can improve the cardiac function of CHF rats by inhibiting the expression of SERCA2a related protein by inhibiting miR-25.
heart failurecompound Xinshuaikang capsuleSERCA2amiR-25-3pexperimental study
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