TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-oxidized cellulose nanofibers (TOCNs)were used as nanofillers for polyvinyl alcohol/waterborne polyurethane electrospun nano-fiber mats in this work.The reinforcing capability of TOCNs was investigated by tensile tests.SEM,IR,TGA and DSC were also carried out in order to characterize the influence of the cellulose nanofibers on electrospun mats.Scanning electron mi-croscopy results showed that TOCNs were well dispersed in PVA/WPU matrix.The reinforced composites had a ca.44% increase in their mechanical properties with addition of only 5wt% of TOCNs.Moreover,The addi-tion of TOCNs improved the thermal stability of PVA/WPU electrospun mats.TOCNs were working as Nano-fillers in the composite.Since PVA,WPU and TOCNs are hydrophilic,non-toxic and biocompatible,and there-fore,these electrospun nano-fiber mats could be used for tissue scaffolding and wound dressing materials,etc.