Preparation and evaluation of catechol modified cold water fish skin gelatin tissue adhesive
Cold water fish skin gelatin(FSGltn)was chosen as the main material,wherein catechol groups(CAT)were linked through quantitative modification to prepare a catechol-modified gelatin(CAT-FSGltn).Then CAT-FSGltn and dextran dialdehyde(DDA)were mixed in situ to prepare a tissue adhesive(CAT-FS-Gltn/DDA)with good adhesion in a wet environment.It was found that the gelation time of CAT-FSGltn/DDA was within 60 s.The fully crosslinked CAT-FSGltn/DDA hydrogel showed minimal mass loss after being im-mersed in PBS solution for over 80 h.When adhered to a rubber film resembling the tissue membrane,the hy-drogel CAT-FSGltn/DDA demonstrated an ability to withstand stretching up to twice its initial length without fracturing.An in vitro model was used to evaluate the compressive strength of the tissue adhesive.The com-pressive strength of CAT-FSGltn/DDA was observed to reach 105.7 mmHg,surpassing the human diastolic pressure.The results indicated that CAT-FSGltn/DDA possessed both strong cohesion and adhesion.Tissue sections showed that CAT-FSGltn/DDA cured for 30 min was closely attached to the casing surface,and the hydrogel/casing interface remained intact after the water flow impact.Cell experiments showed that the survival rate of L929 cells was more than 80％,CAT-FSGltn/DDA hydrogel had good biocompatibility.
cold water fish skin gelatincatecholglucantissue adhesivehydrogel
万方数据
维普
