Cold water fish skin gelatin(FSGltn)was chosen as the main material,wherein catechol groups(CAT)were linked through quantitative modification to prepare a catechol-modified gelatin(CAT-FSGltn).Then CAT-FSGltn and dextran dialdehyde(DDA)were mixed in situ to prepare a tissue adhesive(CAT-FS-Gltn/DDA)with good adhesion in a wet environment.It was found that the gelation time of CAT-FSGltn/DDA was within 60 s.The fully crosslinked CAT-FSGltn/DDA hydrogel showed minimal mass loss after being im-mersed in PBS solution for over 80 h.When adhered to a rubber film resembling the tissue membrane,the hy-drogel CAT-FSGltn/DDA demonstrated an ability to withstand stretching up to twice its initial length without fracturing.An in vitro model was used to evaluate the compressive strength of the tissue adhesive.The com-pressive strength of CAT-FSGltn/DDA was observed to reach 105.7 mmHg,surpassing the human diastolic pressure.The results indicated that CAT-FSGltn/DDA possessed both strong cohesion and adhesion.Tissue sections showed that CAT-FSGltn/DDA cured for 30 min was closely attached to the casing surface,and the hydrogel/casing interface remained intact after the water flow impact.Cell experiments showed that the survival rate of L929 cells was more than 80％,CAT-FSGltn/DDA hydrogel had good biocompatibility.
关键词
冷水鱼皮明胶/儿茶酚/葡聚糖/组织粘合剂/水凝胶
Key words
cold water fish skin gelatin/catechol/glucan/tissue adhesive/hydrogel
维普
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