显微红外光谱法研究豚鼠膝关节软骨下骨的增龄性改变
FTIR Microspectroscopic Investigation of the Age-Related Changes of Subchondral Bone of the Knee in Guinea Pig
杨丽平 1刘建玲 2宋庆慧 1朱嘉 1张万强 1孔焕宇 1赵铁军3
作者信息
- 1. 中国中医科学院望京医院中药药理(骨伤)实验室,北京100700
- 2. 中国人民解放军第二五一医院中心实验室,河北张家口075000
- 3. 中国中医科学院望京医院关节一科,北京 100102
- 折叠
摘要
应用显微FTIR技术原位分析雌性Hartley豚鼠随月龄增加胫骨关节软骨下骨的化学变化.红外光谱测定三种月龄(1月、2月和3月)豚鼠软骨下3个不同吸收颜色的骨小梁区(a,b,c)和中央骨髓区(d).结果显示:(1)随月龄增加,骨小梁总面积增加,与正常骨小梁谱图相似的a区减少,d区波形有逐渐向a区发展的趋势.(2)2月龄和3月龄的b和c区AmideⅢ红移以c区红移显著并且变成肩峰,代表核酸和多糖的吸收峰峰强是a区的7倍.(3)三种月龄的c区均出现了β型糖苷键吸收峰.(4)IAmide/IArnidedeⅡ在2月b区最大,3月各区最小,IAmide/IArnidedeⅡ在2月、3月由a到c依次降低,IAmide/IArnidedeⅡ在2月、3月的b和c区较相应a区高7倍以上.结果符合骨关节炎不同阶段软骨下骨的组织结构及化学组成的变化规律.初步研究表明,显微光谱成像技术结合FTIR可原位分析不同区域的软骨下骨小梁和骨髓的分子组成,为骨关节炎软骨下骨组织病理学的分子水平研究提供了可靠信息.
Abstract
Fourier transform infrared (FTIR) micmspectroscopy was applied to in-situ analyse the chemical change of tibial articular subchondral bone of female Hartley guinea pigs with age increase.Three infrared absorption regions (a,b,c) of trabecular bone and central marrow region of the subchondral bone were measured for guinea pigs of different ages (1 months,2 months and 3 months) using the infrared spectrum.Results show that (1) with months increasing,the total area of trabecular bone is increasing,meanwhile,the region a which is similar to normal trabecular bone spectra is decreasing,and region d waveform has the same trend as region a.(2) In the second and third month,region b & c show amide Ⅲ redshift and the red shift in region c shows a shoulder peak,showing the absorption peak intensity on behalf of nucleic acid and polysaccharide in region b & c is 7 times that in region a.(3)β glycosidic bond absorption peak appears at region c in 3 different old pigs.(4) IamrodeⅢ/Imrode Ⅲ is the highest in region b in the second month but lowest in the third month; Imrode/Imrode reduces from a to c in the second and third month; Ivsmi/Iamrode,in region b & c is 7 times higher than region a in the second and third Month.These results are consistent with the regular pattern of change rule of osteoarthritis subchondral bone's organization structure and chemical composition in different stages.Our primary result illustrated that FTIR microspectmscopy can be used for in-situ analysis of the molecular organization of subchondral trabecular bone and bone marrow.It provides reliable pathology information for osteoarthritis subchondral bone tissue at molecular level.
关键词
显微FTIR/骨关节炎/软骨下骨/骨小梁/化学组成Key words
FTIR microspectroscopy/Osteoarthritis/Subchondral bone/Trabecular bone/Chemical composition引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据