Simultaneous Determination of 6 Neonicotinoid Residues in Soil Using DLLME-HPLC and UV
A simple,cheap and rugged method was developed for simultaneous deter mination of 6 neonicotinoid residues in soil,including imidacloprid,acetamiprid,thiamethoxam,thiacloprid,clothianidin and nitenpyrarm.The soil sample was produced by dispersive liquid-liquid micro-extraction (DLLME) after extracted by the mixed solution of acemnitrile and CH2Cl2 (2 ∶ 1,φ).The analytes were separated by HPLC with Alltima TMC18 column (4.6 mm×250 mm,5 μin) and detected by PDA at 260 nm.External standard method was used for quantification.The results showed that good lineadty was obtained with correlation coefficients between 0.998 2 and 0.999 9 in the range of 0.5～200 μg · L-1.The limits of detection (LODs) were in the range between 0.000 5 and 0.003μg · mL-1 (S/N=3).The method was validated with five soil samples spiked at three fortification levels (0.05,0.1,1.0 mg · kg-1) and recoveries were in the range of 55.3％95.6％ with RSD of 1.47.0％.The effect of clean-up was evaluated by UV spectra and demonstrated that the method established is effective.In conclusion,this method is competent for the simultaneous analysis of 6 neonicotinoid residues in soil
NETL
NSTL
万方数据
维普
