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湿地土壤硝化微生物群落的高光谱研究

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氮循环是土壤生态系统元素循环的重要过程,其中硝化作用对于土壤氮循环有重要影响。硝化作用的主要完成者是硝化微生物群落,土壤微生物是湿地生态系统的重要组成部分,其可以指示湿地生态环境变化,对正确认识湿地生态系统氮循环和湿地污染净化功能具有重要意义。尝试从高光谱遥感技术角度,基于土壤氮素光谱监测机理,探索湿地土壤硝化微生物群落高光谱估算技术,进而为估测其时空分布状况提供新技术途径。研究对硝化作用中两个独立阶段的主要完成者氨氧化细菌和亚硝酸氧化细菌,采用最大可能数法分别计数,并以两者计数测量结果的合计,作为各采样区域土壤硝化微生物的数量值。采用光谱倒数的对数(LR)、光谱一阶微分(FD)、光谱二阶微分(SD)、包络线去除(CR)和光谱波段深度(BD)光谱变换技术,以及基于再抽样(bootstrap)技术的多元逐步回归(SMLR)和偏最小二乘回归(PLSR)建模方法,构建湿地土壤硝化微生物数量和全氮含量估算模型。研究结果表明:在采用 bootstrap SMLR建模方法时,湿地土壤硝化微生物数量和全氮含量的估算波段位置存在一定的相似性(尤其对于原光谱实测数据 R 和 SD 光谱);对于湿地土壤硝化微生物数量和全氮含量的估算,bootstrap PLSR 相比于 bootstrap SMLR 建模方法,具有较高的估算精度;对湿地土壤硝化微生物数量的估算,最高估算精度产生于 SD 光谱变换技术结合bootstrap PLSR建模;对湿地土壤全氮含量的估算,最高估算精度产生于 CR 光谱变换技术结合 bootstrap PLSR建模。
Hyperspectral Study of Estimating Nitrification Microorganism in Wetland Soils
Nitrogen cycle is an important process in the circle of soil ecosystem elements,and nitrification has significant effect on soil nitrogen cycling.The main completer of nitrification is nitrification microbial communities.Soil microorganisms are vital components of wetland ecosystem.They can indicate the variations of wetland ecological environment,and this helps us to have the correct understanding of nitrogen cycle and pollution purification function in wetland ecosystem.This paper tries to study ni-trification microbial communities in wetland soils from the perspective of hyperspectral remote sensing technology,based on the monitoring mechanisms of soil nitrogen spectrum.The study explores hyperspectral estimation techniques for nitrification micro-bial communities in wetland soils,and it can provide a new technical approach to estimate the temporal and spatial distribution of nitrification microbial communities.The study adopted most probable number method (MPN)to count the numbers of ammonia oxidizing bacteria and nitrite oxidizing bacteria respectively,which were main completers of two independent stages in nitrifica-tion.And the total results of both count measurements were used as the values of soil nitrification microorganisms for each sam-pling area.The estimation models of nitrification microorganism and total nitrogen in wetland soils were developed respectively using spectral transformation techniques,such as log-transformed spectra (LR),first derivative (FD),second derivative (SD), continuum removal (CR)and band depth (BD),and modeling methods,such as stepwise multiple linear regression (SMLR)and partial least-squares regression (PLSR)based on the bootstrap technology.The results indicated that the selected estimation bands of nitrification microorganism and total nitrogen were close (especially for original spectral data (R)and SD spectra)when the modeling method of bootstrap SMLR was used.Compared to the bootstrap SMLR,the bootstrap PLSR achieved higher ac-curacies for estimating nitrification microorganism and total nitrogen in wetland soils.The spectral transformation technique of SD combined with the modeling method of bootstrap PLSR yielded the highest estimation accuracy to predict nitrification micro-organism in wetland soils.The CR spectral data combined with bootstrap PLSR produced the highest estimation accuracy to pre-dict total nitrogen content in wetland soils.

Hyperspectral modelsNitrification microorganismWetland soilsBootstrap PLSR

卫亚星、王莉雯

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辽宁师范大学海洋经济与可持续发展研究中心,辽宁 大连 116029

辽宁师范大学自然地理与空间信息科学辽宁省重点实验室,辽宁 大连 116029

辽宁师范大学城市与环境学院,辽宁 大连 116029

高光谱模型 硝化微生物 湿地土壤 再抽样的多元逐步回归

国家自然科学基金教育部人文社会科学研究规划基金

4127142114YJA630064

2016

光谱学与光谱分析
中国光学学会

光谱学与光谱分析

CSTPCDCSCD北大核心SCIEI
影响因子:0.897
ISSN:1000-0593
年,卷(期):2016.36(10)
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