Design,Synthesis and Performance of Fluorescent Probe for Detection of Hg2+With 1,3-Oxathiolane as Receptor
Hg2+is one of the most toxic heavy metal ions,which can cause air,soil,and water pollution,seriously damaging human health.Therefore,developing effective analytical methods to detect Hg2+in environmental systems is particularly important.Fluorescent probes have been widely used to detect Hg2+due to their advantages,such as high sensitivity,good selectivity,fast response time,and real-time online detection.In this paper,a novel"turn-on"fluorescent probe(2-(pyren-1-yl)-1,3-oxathiolane,POX)with 1,3-oxathiolane as receptor was designed and synthesized based on Hg2--promoted deprotection reaction of thioacetal,and 1H NMR,13C NMR,and HRMS characterized its structure.The selectivity,competitiveness,concentration titration,pH titration,time dependence,the limit of detection,and recognition mechanism of POX for the detection of Hg2+in CH3CH2OH/H2O solution were investigated.The results showed that POX could quickly recognize Hg2+in a wide pH range and exhibited high selectivity and sensitivity.Adding Hg2+to the solution of POX resulted in a clear fluorescence emission peak at 386 nm,indicating that POX showed a remarkable"turn-on"fluorescence for Hg2+,and its recognition process was almost unaffected by other metal ions.Fluorescence titration experiments indicated that POX had a good linear response(R2=0.999 4)in the range of Hg2+from 0～6.5 μmol·L-1,with a detection limit of 0.168 μmol·L-1.The RSD of POX for detecting Hg2+in actual water samples was less than 2.92％.The simple synthesis,easy availability of raw materials,and wide pH applicability of POX suggested that itcould be used as a potential tool for the qualitative and quantitative detection of Hg2 in the environment.
NETL
NSTL
万方数据
