Adventitious shoot regeneration from in vitro leaf of Juglans regia cv. Xi-angling
Experiment was conducted with the leaves of walnut cultivar Xiangling as material for studying the best medium for adventitious shoot regeneration under dark culture conditions. The result showed that the optimum dark incubation time was 14 d,and using the adaxial leaf touching medium was better for regeneration. The optimum medium was MS + BA 0.50 mg·L~(-1) +NAA 0.5 mg·L~(-1)+TDZ 1.0 mg·L~(-1)+sucrose 30 g·L~(-1)+ agar 6 g·L~(-1), on this medium in vitro the adventitious shoot frequency of regeneration could reach 60%, and the shoot number per. explant was more than 1.1.
国家科技期刊平台
NSTL
万方数据
维普
