Application of ISSR markers in taxonomy of Chinese wild grape
16 Chinese wild grape species and their relatives were studied by ISSR markers on taxonomy.13 primers selected from 100 primers were used for ISSR amplification. 185 bands were generated which molecular weight ranged from 300 to 2 000 bp, of which 175 bands were polymorphic bands (the percentage of polymorphic band, PPB＝94.6％). According to the results of ISSR amplification, the similarity coefficient were analyzed by NTSYSpc2.10e software, the genetic similarity coefficient was 0.57 to 0.99. The clustering dendrogram was constructed by UPGMA method to explore the taxonomic relationship between 81 Vitis materials. To employ Ampelopsis sinica (Miq.) W. T. Wang. and Vitis rotundifolia as Outgroup,which had genetic similarity coefficient 0.57 and 0.60 with Euvitis, respectively. The 79 Euvitis materials were classified as 22 groups according to the cluster analysis of the PCR amplified products. Most of materials had the same traditional classification but some not. According to the results of ISSR clustering ,we determined the Maoputao 1099 as Vitis ftcifolia Bunge. Whether Yanshanputao 0947 is Vitis yeshanensis J. X. Chen was suspected. We also found inter-specie types existed in 10 Vitis piasezkii Maxim. Materials.
Chinese wild grapeISSRGenetic similarity coefficientTaxonomy
国家科技期刊平台
NSTL
万方数据
维普
