首页|可可球二孢(Botryodiplodia theobromae)细胞壁降解酶对木亡果果实致病作用

可可球二孢(Botryodiplodia theobromae)细胞壁降解酶对木亡果果实致病作用

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为明确可可球二孢(Botryodiplodia theobromae Pat.)产细胞壁降解酶在侵染杧果果实过程中的作用,研究了可可球二孢在离体培养和接种条件下分泌的5种细胞壁降解酶活性变化特点,以及可可球二孢(B.theobromae)、胶孢炭疽菌(Colletotrichum gloeosporioides Penz.)、杧果拟茎点霉(Phomopsismangiferae Ahmad)和小穴壳菌(Dothiorella do-minicana Pet.et Cif.)4种杧果采后主要蒂腐病菌分泌细胞壁降解酶的能力。结果表明,可可球二孢在离体培养条件下或接种杧果果实后均可产生多聚半乳糖醛酸酶(PG)、果胶甲基半乳糖醛酸酶(PMG)、多聚半乳糖醛酸反式消除酶(PGTE)、果胶甲基反式消除酶(PMTE),以及纤维素酶(Cx)。其中PG、PMG和Cx活性较高,PGTE和PMTE活性极低。在大量分泌的3种细胞壁降解酶中,PG和Cx活性高峰出现较早,PMG活性高峰出现较迟。可可球二孢的致病力显著高于胶孢炭疽菌、杧果拟茎点霉和小穴壳菌。其可可球二孢分泌果胶酶的能力也显著高于其他3种病菌。
Pathogenicity of cell wall degrading enzymes produced by Botryodiplodia theobromae against mango fruit
To confirm the effects of cell wall degrading enzymes (CWDEs) produced by Botryodiplodia theobromae Pat. on mango fruits , the activity of 5 CWDEs were examined , and the pathogenicity and the ability of producing CWDEs of four fungal pathogens causing mango stem rot , such as Colletotrichum gloeosporioides Penz. ,Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet.et cif. were studied. B. theobromae could produce polygalacturonase (PG),pectinmethylgalacturonase (PMG), polygalacturonic acid trans-eliminase (PGTE), pectin methyltrans-eliminase (PMTE) and cellulase (Cx.) when cultured in vitro or in vivo . The activity of PG,Cx and PMG were significantly higher than that of PGTE and PMTE. Among three primary CWDEs, the activity peak of PG and Cx appeared earlier and PMG's peak came late. The pathogenicity of B.theobromae was significantly stronger than that of other three pathogens , and the ability of producing pectinase of B .theobromae was remarkably stronger than that of other three pathogens.

Botryodiplodia theobromae Pat.Cell wall degrading enzymePathogenicity

李敏、高兆银、胡美姣、周铄、杨冬平、杨波、易建学、杨凤珍

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中国热带农业科学院环境与植物保护研究所/海南省热带农业有害生物监测与控制重点实验室/海南省热带作物病虫害生物防治工程技术研究中心

海南大学环境与植物保护学院,海南儋州571737

中国热带农业科学院环境与植物保护研究所/海南大学环境与植物保护学院,海南儋州571737

杧果 可可球二孢 细胞壁降解酶 致病力

行业(农业)科研专项中央级公益性科研院所基本科研业务费专项中央级公益性科研院所基本科研业务费专项中央级公益性科研院所基本科研业务费专项

3-44-22009hzs1J0112011hzs1J0272011hzs1J004

2011

果树学报
中国农业科学院郑州果树研究所

果树学报

CSTPCDCSCD北大核心
影响因子:1.486
ISSN:1009-9980
年,卷(期):2011.28(6)
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