Study on two auxin-biosynthesis related genes YUC1 and YUC2 in octoploid and diploid strawberry
[Objective]The study was to elucidate the putative function of the flavin monooxygenases (FMOs) related genes in the strawberry.[Method]Two YUC-type FMO genes FvYUC1-2 were isolated from woodland strawberry (Fragaria vesca var.Heilongjiang3#) based on a homologous cloning strategy,and the molecular characteristics and expression patterns of YUC homologous genes were comparatively analyzed in octoploid and diploid strawberries by using DNAMAN software and RT-PCR.[Result]The results of homologous analysis showed that the sequence identity of YUC gene between these two strawberries was 99.31％ for YUC1 at amino acid level, and 99.02％ for YUC2, respectively.However, FvYUC1 shared only 61.6％ sequence identity with FvYUC2.Quantitative RT-PCR revealed that although YUC1 and YUC2 expressed in all tested six tissues of 'Jiuxiang' and 'Heilongjiang3#', their expression patterns differed greatly.YUC1 was predominantly expressed in the young leaves of woodland strawberry,while in the roots of cultivated strawberry.YUC2 was preferentially expressed in the stolon of woodland strawberry, while in the small green fruit of cultivated strawberry.[Conclusion]These results suggested that the main functions of YUC gene might differ greatly in woodland and cultivated strawberry, though their sequences were highly conserved.Therefore, functional studies in woodland strawberry and cultivated strawberry need to be integrated carefully.
国家科技期刊平台
NSTL
万方数据
维普
